Biotin Conjugated Mouse FGF-21 Recombinant Rabbit Monoclonal Antibody [PSH15-27]
cat.: HA723733B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH15-27 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse FGF21 aa 29-210 (HA211166). |
| Positive control: | Recombinant Mouse FGF-21 protein (HA211166). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH15-26] to Mouse FGF-21 antibody (Capture) (HA723731) and Recombinant Mouse FGF-21 protein (HA211166) as the standard. The reference range value is 9.8-2,500 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q9JJN1 Mouse |
| Alternative names: | FGF 21 FGF-21 Fgf21 FGF21_HUMAN FGFL Fibroblast growth factor 21 PRO10196 UNQ3115 |
Images
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Fig1:
Sandwich ELISA analysis of Mouse FGF-21 matched pair antibodies Capture: HA723731, Mouse FGF-21 Rabbit mAb [PSH15-26] Detector: HA723732, Mouse FGF-21 Rabbit mAb [PSH15-27] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723731) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse FGF-21 protein (HA211166) starting from 1250 pg/ml to 0 pg/ml and detect antibody (HA723732, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native FGF-21 in mouse samples. Capture: HA723731, Mouse FGF-21 Rabbit mAb [PSH15-26] Detector: HA723732, Mouse FGF-21 Rabbit mAb [PSH15-27] Interpolated concentration of native FGF-21 was measured in duplicate at different sample concentrations and interpolated from the FGF-21 standard curves. Undiluted samples were 100% Mouse serum and 100% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean FGF-21 concentration was determined to be 1,196 pg/mL in mouse serum. There was no detectable signal in 3T3-L1 cell supernatant. |
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Fig3:
Interpolated concentrations of spiked FGF-21 in cell culture media samples. Capture: HA723731, Mouse FGF-21 Rabbit mAb [PSH15-26] Detector: HA723732, Mouse FGF-21 Rabbit mAb [PSH15-27] The concentrations of FGF-21 were measured in duplicates, interpolated from the FGF-21 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.