SMC5 Recombinant Rabbit Monoclonal Antibody [PSH15-29]
cat.: HA723735
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat, Monkey
Applications: WB, IF-Cell, IHC-P
Clonality: Monoclonal
Clone number: PSH15-29
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 129 kDa
Isotype: IgG
Immunogen: Recombinant protein within human SMC5 aa 901-1,101.
Positive control: HeLa cell lysate, Jurkat cell lysate, U-2 OS cell lysate, HEK-293 cell lysate, COS-1 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate, C6, mouse testis tissue, rat testis tissue.
Subcellular location: Nucleus, Chromosome, Nucleus, PML body, telomere.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
1:5,000
1:100
1:200
Uniprot #: SwissProt: Q8IY18 Human | Q8CG46 Mouse
Entrez Gene: 293967 Rat
Alternative names: hSMC 5 hSMC5 KIAA0594 MLZ-453 OTTHUMP00000063367 SMC 5 SMC 5 structural maintenance of chromosomes 5 like 1 SMC protein 5 SMC-5 smc5 SMC5 L1 SMC5 protein SMC5 structural maintenance of chromosomes 5 like 1 SMC5_HUMAN SMC5L1 Structural maintenance of chromosomes 5 Structural maintenance of chromosomes 5 like 1 Structural maintenance of chromosomes protein 5
Images
HA723735_1.jpg Fig1: Western blot analysis of SMC5 on different lysates with Rabbit anti-SMC5 antibody (HA723735) at 1/5,000 dilution.

Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: Jurkat cell lysate (20 µg/Lane)
Lane 3: U-2 OS cell lysate (20 µg/Lane)
Lane 4: HEK-293 cell lysate (20 µg/Lane)
Lane 5: COS-1 cell lysate (20 µg/Lane)
Lane 6: NIH/3T3 cell lysate (20 µg/Lane)
Lane 7: C6 cell lysate (20 µg/Lane)
Lane 8: Mouse testis tissue lysate (40 µg/Lane)
Lane 9: Rat testis tissue lysate (40 µg/Lane)

Predicted band size: 129 kDa
Observed band size: 129 kDa

Exposure time: 25 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723735) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA723735_2.jpg Fig2: Immunocytochemistry analysis of C6 cells labeling SMC5 with Rabbit anti-SMC5 antibody (HA723735) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SMC5 antibody (HA723735) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
HA723735_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-SMC5 antibody (HA723735) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723735) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA723735_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-SMC5 antibody (HA723735) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723735) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.