| Product Type: | Recombinant Rabbit multiclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clone number: | PSH15-35 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 25 kDa |
| Isotype: | IgG |
| Positive control: | Human serum tissue lysate, Human plasma tissue lysate, Mouse serum tissue lysate, Mouse plasma tissue lysate, Rat plasma tissue lysate. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: P02741 Human | P14847 Mouse | P48199 Rat |
| Alternative names: | Pentraxin 1, short C reactive protein C reactive protein pentraxin related C-reactive protein(1-205) CRP CRP_HUMAN MGC88244 Pentraxin 1 PTX 1 PTX1 |
|
Fig1:
Western blot analysis of C Reactive Protein on different lysates with Rabbit anti-C Reactive Protein antibody (HA723739) at 1/5,000 dilution. Lane 1: Human serum tissue lysate Lane 2: Human plasma tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 25 kDa Observed band size: 25 kDa Exposure time: 7 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723739) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of C Reactive Protein on different lysates with Rabbit anti-C Reactive Protein antibody (HA723739) at 1/5,000 dilution. Lane 1: Mouse serum tissue lysate Lane 2: Mouse plasma tissue lysate Lane 3: Rat plasma tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 25 kDa Observed band size: 25 kDa Exposure time: 7 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723739) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |