Biotin Conjugated Human PD-L1 Recombinant Rabbit Monoclonal Antibody [PSH15-37]
cat.: HA723742B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Cynomolgus monkey |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH15-37 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human PD-L1 aa 19-238 (HA210954). |
| Positive control: | Recombinant Human PD-L1 protein (HA210954). |
| Subcellular location: | Cell membrane, Early endosome membrane, Recycling endosome membrane, Nucleus. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH15-36] to Human PD-L1R antibody (Capture) (HA723740) and Recombinant Human PD-L1 protein (HA210954) as the standard. The reference range value is 7.8-2,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q9NZQ7 Human |
| Alternative names: | B7 H B7 H1 B7 homolog 1 B7-H1 B7H B7H1 CD 274 CD-274 CD274 CD274 antigen CD274 molecule MGC142294 MGC142296 OTTHUMP00000021029 PD L1 PD-L1 PD1L1_HUMAN PDCD1 ligand 1 PDCD1L1 PDCD1LG1 PDL 1 PDL1 Programmed cell death 1 ligand 1 Programmed death ligand 1 RGD1566211 |
Images
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Fig1:
Sandwich ELISA analysis of Human PD-L1 matched pair antibodies Capture: HA723740, Human PD-L1 Rabbit mAb [PSH15-36] Detector: HA723741, Human PD-L1 Rabbit mAb [PSH15-37] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723740) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human PD-L1 protein (HA210954) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723741, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native PD-L1 in A549 Cell extract samples based on a 1000 µg/ml extract load. Capture: HA723740, Human PD-L1 Rabbit mAb [PSH15-36] Detector: HA723741, Human PD-L1 Rabbit mAb [PSH15-37] Interpolated concentration of native PD-L1 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean PD-L1 concentration was determined to be 1108 pg/mL in A549 treated Cell extract and undetectable in A549 untreated Cell extract. |
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Fig3:
Interpolated concentrations of spiked PD-L1 in cell culture media samples. Capture: HA723740, Human PD-L1 Rabbit mAb [PSH15-36] Detector: HA723741, Human PD-L1 Rabbit mAb [PSH15-37] The concentrations of PD-L1 were measured in duplicates, interpolated from the PD-L1 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.