KLF5 Recombinant Rabbit Monoclonal Antibody [PSH15-42]
cat.: HA723748
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: IF-Cell, FC
Clonality: Monoclonal
Clone number: PSH15-42
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 51 kDa
Isotype: IgG
Immunogen: Recombinant protein within human KLF5 aa 81-380.
Positive control: HeLa, NIH/3T3, C6.
Subcellular location: Nucleus.
Recommended Dilutions:
  IF-Cell
  FC
1:2,000
1:1,000
Uniprot #: SwissProt: Q13887 Human | Q9Z0Z7 Mouse
Entrez Gene: 84410 Rat
Alternative names: Basic transcription element binding protein 2 Basic transcription element-binding protein 2 BTE binding protein 2 BTE-binding protein 2 BTEB 2 BTEB2 CKLF Colon krueppel like factor Colon krueppel-like factor GC box binding protein 2 GC-box-binding protein 2 IKLF Intestinal enriched krueppel like factor Intestinal-enriched krueppel-like factor KLF 5 Klf5 KLF5_HUMAN Krueppel like factor 5 Krueppel-like factor 5 Kruppel like factor 5 intestinal Transcription factor BTEB 2 Transcription factor BTEB2
Images
HA723748_1.jpg Fig1: Immunocytochemistry analysis of HeLa cells labeling KLF5 with Rabbit anti-KLF5 antibody (HA723748) at 1/2,000 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-KLF5 antibody (HA723748) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
HA723748_2.jpg Fig2: Immunocytochemistry analysis of NIH/3T3 cells labeling KLF5 with Rabbit anti-KLF5 antibody (HA723748) at 1/2,000 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-KLF5 antibody (HA723748) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
HA723748_3.jpg Fig3: Immunocytochemistry analysis of C6 cells labeling KLF5 with Rabbit anti-KLF5 antibody (HA723748) at 1/2,000 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-KLF5 antibody (HA723748) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
HA723748_4.jpg Fig4: Flow cytometric analysis of HeLa cells labeling KLF5.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA723748, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
HA723748_5.jpg Fig5: Flow cytometric analysis of NIH/3T3 cells labeling KLF5.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA723748, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
HA723748_6.jpg Fig6: Flow cytometric analysis of C6 cells labeling KLF5.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA723748, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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