WWOX Recombinant Rabbit Monoclonal Antibody [PSH15-44]
cat.: HA723750
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Green monkey |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH15-44 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 47 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human WWOX aa 1-414. |
| Positive control: | MCF7 cell lysate, HepG2 cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, C6 cell lysate, COS-1 cell lysate, Rat brain tissue lysate. |
| Subcellular location: | Cytoplasm, Nucleus, Mitochondrion, Golgi apparatus, Lysosome. |
| Recommended Dilutions:
WB IP |
1:10,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q9NZC7 Human | Q91WL8 Mouse Entrez Gene: 292041 Rat |
| Alternative names: | 5330426P09Rik 9030416C10Rik Aberrant WW domain-containing oxidoreductase D16S432E EC 1.1.1.- EIEE28 FOR FRA16D Fragile site FRA16D oxidoreductase Fragile site FRA16D Oxireductase HHCMA56 MGC55975 PRO0128 Putative oxidoreductase SCAR12 SDR41C1 Short chain dehydrogenase/reductase family 41C, member 1 WOX1 WW domain containing oxidoreductase WW domain-containing oxidoreductase WW domain-containing protein WWOX wwox WWOX_HUMAN zgc:55975 |
Images
|
Fig1:
Western blot analysis of WWOX on different lysates with Rabbit anti-WWOX antibody (HA723750) at 1/10,000 dilution. Lane 1: MCF7 cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: HeLa cell lysate (20 µg/Lane) Lane 4: NIH/3T3 cell lysate (20 µg/Lane) Lane 5: C6 cell lysate (20 µg/Lane) Lane 6: COS-1 cell lysate (20 µg/Lane) Lane 7: Rat brain tissue lysate (30 µg/Lane) Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723750) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
WWOX was immunoprecipitated from 0.2 mg MCF7 cell lysate with HA723750 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723750 at 1/10,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: MCF7 cell lysate (input) Lane 2: HA723750 IP in MCF7 cell lysate Lane 3: Rabbit IgG instead of HA723750 in MCF7 cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 3 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.