Total OXPHOS Antibody Cocktail Recombinant Rabbit Multiclonal Antibody [PSH15-50]
cat.: HA723756
| Product Type: | Recombinant Rabbit multiclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clone number: | PSH15-50 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 18/22/29/48/54 kDa |
| Isotype: | IgG |
| Positive control: | HEK-293 cell lysate, HeLa cell lysate, A549 cell lysate, K-562 cell lysate, MCF7 cell lysate, human breast carcinoma tissue, human liver tissue. |
| Subcellular location: | Mitochondrion inner membrane. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:200 |
| Uniprot #: | SwissProt: O95169 Human | P00403 Human | P21912 Human | P22695 Human | P25705 Human |
| Alternative names: | ASHI ATP synthase subunit alpha Atp5a1 ATP5AL2 COII Complex I ASHI subunit COX 2 COX II COX2 COXII CWS2 Cytochrome b c1 complex subunit 2 mitochondrial Cytochrome c oxidase II EC 1.10.2.2 FLJ92337 Iron sulfur subunit Iron sulfur subunit of complex II MC3DN5 MC5DN4 mitochondrial MT CO2 MTCO2 NADH dehydrogenase (ubiquinone) 1 beta subcomplex 8 19kDa NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 mitochondrial PGL 4 PGL4 QCR2 SDH 1 SDH1 SDH2 SDH2, homolog of SDHIP Succinate dehydrogenase [ubiquinone] iron sulfur protein mitochondrial Succinate dehydrogenase [ubiquinone] iron sulfur subunit succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial Succinate Dehydrogenase 1 Iron Sulfur Subunit Succinate Dehydrogenase 2, S. cerevisiae, homolog of Succinate dehydrogenase complex iron sulfur subunit B Succinate dehydrogenase complex subunit B iron sulfur Ubiquinol cytochrome c reductase com...... |
Images
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Fig1:
Western blot analysis of Total OXPHOS Antibody Cocktail on different lysates with Rabbit anti-Total OXPHOS Antibody Cocktail antibody (HA723756) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HeLa cell lysate Lane 3: A549 cell lysate Lane 4: K-562 cell lysate Lane 5: MCF7 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 18/22/29/48/54 kDa Observed band size: 18/22/29/48/54 kDa Exposure time: 15 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723756) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Total OXPHOS Antibody Cocktail antibody (HA723756) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723756) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Total OXPHOS Antibody Cocktail antibody (HA723756) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723756) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.