BCKDHA Recombinant Rabbit Monoclonal Antibody [PSH15-62]
cat.: HA723769
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Green monkey |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH15-62 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 50 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human BCKDHA aa 301-350. |
| Positive control: | HeLa cell lysate, HEK-293 cell lysate, HepG2 cell lysate, A20 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, COS-1 cell lysate, HepG2, NIH/3T3, human kidney tissue, human liver tissue, rat kidney tissue, HeLa. |
| Subcellular location: | Mitochondrion matrix. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:20,000 1:10,000 1:5,000-1:8,000 1:1,000 |
| Uniprot #: | SwissProt: P12694 Human | P50136 Mouse | P11960 Rat |
| Alternative names: | Branched chain alpha keto acid dehydrogenase E1 component alpha polypeptide FLJ45695 OVD1A 2 oxoisovalerate dehydrogenase (lipoamide) 2 oxoisovalerate dehydrogenase subunit alpha, mitochondrial 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial BCKDE1A BCKDH E1 alpha BCKDH E1-alpha BCKDHA Branched chain alpha keto acid dehydrogenase E1 component alpha chain Branched chain keto acid dehydrogenase E1 alpha polypeptide Branched chain keto acid dehydrogenase E1, alpha polypeptide (maple syrup urine disease) Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain MSU MSUD1 ODBA_HUMAN |
Images
|
Fig1:
Western blot analysis of BCKDHA on different lysates with Rabbit anti-BCKDHA antibody (HA723769) at 1/20,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: HepG2 cell lysate Lane 4: A20 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: C6 cell lysate Lane 7: COS-1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 50 kDa Observed band size: 48 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723769) at 1/20,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of HepG2 cells labeling BCKDHA with Rabbit anti-BCKDHA antibody (HA723769) at 1/10,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-BCKDHA antibody (HA723769) at 1/10,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Counterstained with Mitotracker. Nuclear DNA was labelled in blue with DAPI. |
|
Fig3:
Immunocytochemistry analysis of NIH/3T3 cells labeling BCKDHA with Rabbit anti-BCKDHA antibody (HA723769) at 1/10,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-BCKDHA antibody (HA723769) at 1/10,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Counterstained with Mitotracker. Nuclear DNA was labelled in blue with DAPI. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-BCKDHA antibody (HA723769) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723769) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-BCKDHA antibody (HA723769) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723769) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig6:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-BCKDHA antibody (HA723769) at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723769) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig7:
Flow cytometric analysis of HeLa cells labeling BCKDHA. Cells were fixed and permeabilized. Then stained with the primary antibody (HA723769, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
|
Fig8:
Flow cytometric analysis of NIH/3T3 cells labeling BCKDHA. Cells were fixed and permeabilized. Then stained with the primary antibody (HA723769, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.