IL-1 alpha Recombinant Rabbit Monoclonal Antibody [PSH15-66]
cat.: HA723773
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH15-66 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 31 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse IL-1 alpha aa 115-270. |
| Positive control: | RAW264.7 cells treated with 10μg/mL LPS for 8 hours. |
| Subcellular location: | Nucleus, Cytoplasm, Secreted. |
| Recommended Dilutions:
IF-Cell FC |
1:3,000 1:1,000 |
| Uniprot #: | SwissProt: P01582 Mouse |
| Alternative names: | BAF FAF Hematopoietin 1 Hematopoietin-1 IL 1 alpha IL 1A IL-1 alpha Il-1a IL1 ALPHA IL1 IL1A IL1A_HUMAN IL1F1 Interleukin 1 alpha Interleukin-1 alpha Interleukin1 alpha LAF LEM Preinterleukin 1 alpha Pro interleukin 1 alpha |
Images
|
Fig1:
Immunocytochemistry analysis of RAW264.7 cells untreated / treated with 10μg/mL LPS for 8 hours labeling IL-1 alpha with Rabbit anti-IL-1 alpha antibody (HA723773) at 1/3,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IL-1 alpha antibody (HA723773) at 1/3,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
|
Fig2:
Flow cytometric analysis of RAW264.7 cells untreated (left) / treated with 10μg/mL LPS for 8 hours (right) labeling IL-1 alpha. Cells were fixed and permeabilized. Then stained with the primary antibody (HA723773, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.