Biotin Conjugated Mouse G-CSF Recombinant Rabbit Monoclonal Antibody [PSH15-75]
cat.: HA723779B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH15-75 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse G-CSF aa 31-208 (HA210972). |
| Positive control: | Recombinant Mouse G-CSF protein (HA210972). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH15-74] to Mouse G-CSF antibody (Capture) (HA723777) and Recombinant Mouse G-CSF protein (HA210972) as the standard. The reference range value is 15.6-4,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P09920 Mouse |
| Alternative names: | C17orf33 Colony stimulating factor 3 (granulocyte) Colony stimulating factor 3 CSF 3 CSF beta CSF3 CSF3_HUMAN CSF3OS Csfg Filgrastim G-CSF GCSA GCSF Granulocyte colony stimulating factor Granulocyte colony-stimulating factor Lenograstim Macrophage granulocyte inducer 2 MGC45931 MGI 2 Pluripoietin |
Images
|
Fig1:
Sandwich ELISA analysis of Mouse G-CSF matched pair antibodies Capture: HA723777, Mouse G-CSF Rabbit mAb [PSH15-74] Detector: HA723778, Mouse G-CSF Rabbit mAb [PSH15-75] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723777) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse G-CSF protein (HA210972) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723778, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native G-CSF in Raw264.7 LPS stimulated cell culture supernatant. Capture: HA723777, Mouse G-CSF Rabbit mAb [PSH15-74] Detector: HA723778, Mouse G-CSF Rabbit mAb [PSH15-75] Raw264.7 cells were 24 hours serum starved and continued to culture either in the absence or in the presence of 5 µg/ml LPS for 48 hours. The concentrations of G-CSF measured in duplicate and interpolated from the G-CSF standard curve and corrected for sample dilution. Undiluted samples are as follows: unstimulated 100% and stimulated 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean G-CSF concentration was determined to be 1,277 pg/ml in LPS stimulated Raw264.7 cell culture supernatant and undetectable in the unstimulated Raw264.7 control. |
|
Fig3:
Interpolated concentrations of spiked G-CSF in mouse cell culture media samples. Capture: HA723777, Mouse G-CSF Rabbit mAb [PSH15-74] Detector: HA723778, Mouse G-CSF Rabbit mAb [PSH15-75] The concentrations of G-CSF were measured in duplicates, interpolated from the G-CSF standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.