Mouse MDC/CCL22 Recombinant Rabbit Monoclonal Antibody [PSH15-78]
cat.: HA723783
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH15-78 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse MDC/CCL22 aa 25-92 (HA211072). |
| Positive control: | Recombinant Mouse MDC/CCL22 protein (HA211072). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH15-77] to Mouse MDC/CCL22 antibody (Detector) (HA723781) and Recombinant Mouse MDC/CCL22 protein (HA211072) as the standard. The reference range value is 2-500 pg/mL. |
| Uniprot #: | SwissProt: O88430 Mouse |
| Alternative names: | A 152E5.1 ABCD 1 ABCD1 C C motif chemokine 22 CC chemokine STCP 1 CC chemokine STCP-1 ccl 22 Ccl22 CCL22_HUMAN Chemokine (C C motif) ligand 22 DC/B CK DCBCK Macrophage-derived chemokine MDC MDC(1-69) MDC(7-69) MGC34554 SCYA22 Small inducible cytokine subfamily A (Cys Cys) member 22 Small inducible cytokine subfamily A, member 22 Small-inducible cytokine A22 STCP 1 STCP1 Stimulated T cell chemotactic protein 1 Stimulated T-cell chemotactic protein 1 |
Images
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Fig1:
Sandwich ELISA analysis of mouse CCL22 matched pair antibodies Capture: HA723783, Mouse MDC/CCL22 Rabbit mAb [PSH15-78] Detector: HA723781, Mouse MDC/CCL22 Rabbit mAb [PSH15-77] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody MR240604-AA1341-1-1H9 or MR240604-AA1341-1-1C11 diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted mouse CCL22 protein (HA211072) starting from 500 pg/ml to 0 pg/ml and detect antibody MR240604-AA1341-1-1E2 -Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native CCL22 in mouse spleen cell supernatant treated or untreated with LPS for 48 hours. Capture: HA723783, Mouse MDC/CCL22 Rabbit mAb [PSH15-78] Detector: HA723781, Mouse MDC/CCL22 Rabbit mAb [PSH15-77] Interpolated concentration of native CCL22 was measured in duplicate at different sample concentrations and interpolated from the CCL22 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CCL22 concentration was determined to be 202 pg/mL in mouse spleen treated cell supernatant, 25 pg/mL in neat untreated mouse spleen cell supernatant. |
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Fig3:
Interpolated concentrations of native CCL22 in J774A.1 cell supernatant treated or untreated with PHA and PMA for 72 hours. Capture: HA723783, Mouse MDC/CCL22 Rabbit mAb [PSH15-78] Detector: HA723781, Mouse MDC/CCL22 Rabbit mAb [PSH15-77] Interpolated concentration of native CCL22 was measured in duplicate at different sample concentrations and interpolated from the CCL22 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CCL22 concentration was determined to be 525 pg/mL in neat J774A.1 treated supernatant, 17 pg/mL in neat untreated J774A.1 supernatant. |
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Fig4:
Interpolated concentrations of spiked CCL22 in cell culture media samples. Capture: HA723783, Mouse MDC/CCL22 Rabbit mAb [PSH15-78] Detector: HA723781, Mouse MDC/CCL22 Rabbit mAb [PSH15-77] The concentrations of CCL22 were measured in duplicates, interpolated from the CCL22 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.