Mouse MMP-9 Recombinant Rabbit Monoclonal Antibody [PSH15-81]
cat.: HA723787
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH15-81 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse MMP-9 aa 20-730 (HA211045). |
| Positive control: | Recombinant Mouse MMP-9 protein (HA211045). |
| Subcellular location: | Secreted, extracellular space, extracellular matrix. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH15-80] to Mouse MMP-9 antibody (Capture) (HA723785) and Recombinant Mouse MMP-9 protein (HA211045) as the standard. The reference range value is 23.4-6,000 pg/mL. |
| Uniprot #: | SwissProt: P41245 Mouse |
| Alternative names: | 82 kDa matrix metalloproteinase-9 92 kDa gelatinase 92 kDa type IV collagenase CLG 4B CLG4B Collagenase Type 4 beta Collagenase type IV 92 KD EC 3.4.24.35 Gelatinase 92 KD Gelatinase B Gelatinase beta GelatinaseB GELB Macrophage gelatinase MANDP2 Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) Matrix Metalloproteinase 9 MMP 9 MMP-9 MMP9 MMP9_HUMAN Type V collagenase |
Images
|
Fig1:
Sandwich ELISA analysis of Mouse MMP-9 matched pair antibodies Capture: HA723785, Mouse MMP-9 Rabbit mAb [PSH15-80] Detector: HA723787, Mouse MMP-9 Rabbit mAb [PSH15-81] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723785) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse MMP-9 protein (HA211045) starting from 6,000 pg/ml to 0 pg/ml and detect antibody (HA723787, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native MMP-9 in mouse serum samples. Capture: HA723785, Mouse MMP-9 Rabbit mAb [PSH15-80] Detector: HA723787, Mouse MMP-9 Rabbit mAb [PSH15-81] The concentrations of MMP-9 were measured in duplicates, interpolated from the MMP-9 standard curve and corrected for sample dilution. Undiluted samples are mouse serum 2%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP-9 concentration was determined to be 370.1 ng/ml in mouse serum. |
|
Fig3:
Interpolated concentrations of native MMP-9 in mouse LPS unstimulated and stimulated J774A.1 cell culture supernatant. Capture: HA723785, Mouse MMP-9 Rabbit mAb [PSH15-80] Detector: HA723787, Mouse MMP-9 Rabbit mAb [PSH15-81] J774A.1 cells were stimulated with 2.5 µg/ml LPS or vehicle control and incubated for 1 day. The concentrations of MMP-9 measured in duplicate and interpolated from the MMP-9 standard curve and corrected for sample dilution. Undiluted samples are as follows: unstimulated 50% and stimulated 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP-9 concentration was determined to be 21,179 pg/ml in LPS stimulated J774A.1 cell culture supernatant and undetectable in the unstimulated J774A.1 control. |
|
Fig4:
Interpolated concentrations of spiked MMP-9 in mouse cell culture media samples. Capture: HA723785, Mouse MMP-9 Rabbit mAb [PSH15-80] Detector: HA723787, Mouse MMP-9 Rabbit mAb [PSH15-81] The concentrations of MMP-9 were measured in duplicates, interpolated from the MMP-9 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.