Biotin Conjugated Human Endostatin Recombinant Rabbit Monoclonal Antibody [PSH15-83]
cat.: HA723791B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH15-83 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Endostatin aa 1,572-1,754 (HA211001). |
| Positive control: | Recombinant Human Endostatin protein (HA211001). |
| Subcellular location: | Secreted, extracellular space, extracellular matrix, basement membrane. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH15-82] to Human Endostatin antibody (Capture) (HA723789) and Recombinant Human Endostatin protein (HA211001) as the standard. The reference range value is 19.5-5,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P39060 Human |
| Alternative names: | Collagen alpha-1(XVIII) chain Endostatin Non-collagenous domain 1 (NC1) COL18A1 |
Images
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Fig1:
Sandwich ELISA analysis of Human Endostatin matched pair antibodies Capture: HA723789, Human Endostatin Rabbit mAb [PSH15-82] Detector: HA723790, Human Endostatin Rabbit mAb [PSH15-83] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723789) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Endostatin protein (HA211001) starting from 5000 pg/ml to 0 pg/ml and detect antibody (HA723790, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native Endostatin in human samples. Capture: HA723789, Human Endostatin Rabbit mAb [PSH15-82] Detector: HA723790, Human Endostatin Rabbit mAb [PSH15-83] Interpolated concentration of native Endostatin was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Endostatin concentration was determined to be 4,239 pg/ml in HepG2 Cell Supernatant . There was no detectable signal in U-937 cell culture supernatant. |
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Fig3:
Interpolated concentrations of spiked Endostatin in cell culture media samples. Capture: HA723789, Human Endostatin Rabbit mAb [PSH15-82] Detector: HA723790, Human Endostatin Rabbit mAb [PSH15-83] The concentrations of Endostatin were measured in duplicates, interpolated from the Endostatin standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.