Estriol Recombinant Rabbit Monoclonal Antibody [PSH15-86]
cat.: HA723795
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH15-86 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Subcellular location: | Secreted. |
| Alternative names: | E3 |
Images
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Fig1: Indirect ELISA analysis of Estriol was performed by coating wells of a 96-well plate with 50 µl per well of Estriol-BSA antigen diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with StartingBlock blocking buffer, and incubated with 50 µl per well of an Estriol antibody starting at a concentration of 4 µg/mL and serially diluting it to a concentration of 3.9 ng/mL for 2 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1/10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2: Competitive ELISA analysis of Estriol / Estradiol / Estrone / 16-Epiestriol was performed by coating wells of a 96-well plate with 50 µl per well of Estriol-BSA antigen diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. The wells of the plate were washed, blocked with StartingBlock blocking buffer, and then incubated with 50 µL per well of an Estriol antibody at a concentration of 4 µg/mL along with Estriol / Estradiol / Estrone / 16-Epiestriol / PBS buffer only for 2 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1/10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.