Biotin Conjugated Human Granzyme B Recombinant Rabbit Monoclonal Antibody [PSH15-99]
cat.: HA723804B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH15-99 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Granzyme B aa 19-247 (HA211076). |
| Positive control: | Recombinant Human Granzyme B protein (HA211076). |
| Subcellular location: | Secreted, Cytolytic granule. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH15-98] to Human Granzyme B antibody (Capture) (HA723803) and Recombinant Human Granzyme B protein (HA211076) as the standard. The reference range value is 78.1-10,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P10144 Human |
| Alternative names: | C11 Cathepsin G like 1 Cathepsin G-like 1 CCPI CGL 1 CGL1 CSP B CSPB CTLA 1 CTLA-1 CTLA1 CTSGL1 Cytotoxic serine protease B Cytotoxic T lymphocyte associated serine esterase 1 Cytotoxic T lymphocyte proteinase 2 Cytotoxic T-lymphocyte proteinase 2 Fragmentin 2 Fragmentin-2 GRAB_HUMAN Granzyme 2 Granzyme B (granzyme 2, cytotoxic T lymphocyte associated serine esterase 1) Granzyme B Granzyme-2 GranzymeB GRB Gzmb Hlp Human lymphocyte protein Lymphocyte protease Protease, serine, B SECT T cell serine protease 1 3E T cell serine protease 1-3E T-cell serine protease 1-3E |
Images
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Fig1:
Sandwich ELISA analysis of Human Granzyme B matched pair antibodies Capture: HA723803, Human Granzyme B Rabbit mAb [PSH15-98] Detector: HA723804, Human Granzyme B Rabbit mAb [PSH15-99] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723803) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Granzyme B protein (HA211076) protein starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA723804, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native Granzyme B in NK-92 and Jurkat cell culture supernatant. Capture: HA723803, Human Granzyme B Rabbit mAb [PSH15-98] Detector: HA723804, Human Granzyme B Rabbit mAb [PSH15-99] The concentrations of Granzyme B were measured in duplicates, interpolated from the Granzyme B standard curve and corrected for sample dilution. Undiluted samples are NK-92 cell culture supernatant 10% and Jurkat cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Granzyme B concentration was determined to be 45,446 pg/ml in NK-92 cell culture supernatant and undetectable in Jurkat cell culture supernatant. |
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Fig3:
Interpolated concentrations of spiked Granzyme B in human cell culture media samples. Capture: HA723803, Human Granzyme B Rabbit mAb [PSH15-98] Detector: HA723804, Human Granzyme B Rabbit mAb [PSH15-99] The concentrations of Granzyme B were measured in duplicates, interpolated from the Granzyme B standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.