Mouse Resistin Recombinant Rabbit Monoclonal Antibody [PSH16-04]
cat.: HA723809
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH16-04 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse Resistin aa 21-114 (HA211042). |
| Positive control: | Recombinant Mouse Resistin protein (HA211042). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH16-03] to Mouse Resistin antibody (Capture) (HA723808) and Recombinant Mouse Resistin protein (HA211042) as the standard. The reference range value is 15.6-4,000 pg/mL. |
| Uniprot #: | SwissProt: Q99P87 Mouse |
| Alternative names: | Adipose tissue specific secretory factor Adipose tissue-specific secretory factor ADSF C/EBP epsilon regulated myeloid specific secreted cysteine rich protein C/EBP epsilon regulated myeloid specific secreted cysteine rich protein precursor 1 C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein CG5403 Cysteine rich secreted protein A12 alpha like 2 Cysteine rich secreted protein FIZZ3 Cysteine-rich secreted protein A12-alpha-like 2 Cysteine-rich secreted protein FIZZ3 dri FIZZ 3 FIZZ3 Found in inflammatory zone 3 HXCP 1 HXCP1 MGC126603 MGC126609 PRO1199 Resistin Resistin delta2 RETN 1 RETN RETN1 RSTN UNQ407 XCP 1 XCP1 |
Images
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Fig1:
Sandwich ELISA analysis of Mouse Resistin matched pair antibodies Capture: HA723808, Mouse Resistin Rabbit mAb [PSH16-03] Detector: HA723809, Mouse Resistin Rabbit mAb [PSH16-04] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723808) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Resistin protein (HA211042) starting from 4000 pg/ml to 0 pg/ml and detect antibody (HA723809, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native Resistin in Mouse serum. Capture: HA723808, Mouse Resistin Rabbit mAb [PSH16-03] Detector: HA723809, Mouse Resistin Rabbit mAb [PSH16-04] Interpolated concentration of native Resistin was measured in duplicate at different sample concentrations and interpolated from the RETN standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Resistin concentration was determined to be 44636 pg/ml in Mouse serum. |
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Fig3:
Interpolated concentrations of spiked Resistin in cell culture media samples. Capture: HA723808, Mouse Resistin Rabbit mAb [PSH16-03] Detector: HA723809, Mouse Resistin Rabbit mAb [PSH16-04] The concentrations of Resistin were measured in duplicates, interpolated from the Resistin standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.