HRP Conjugated Mouse Resistin Recombinant Rabbit Monoclonal Antibody [PSH16-04]
cat.: HA723809H
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH16-04 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse Resistin aa 21-114 (HA211042). |
| Positive control: | Recombinant Mouse Resistin protein (HA211042). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH16-03] to Mouse Resistin antibody (Capture) (HA723808) and Recombinant Mouse Resistin protein (HA211042) as the standard. The reference range value is 23.4-100,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q99P87 Mouse |
| Alternative names: | Adipose tissue specific secretory factor Adipose tissue-specific secretory factor ADSF C/EBP epsilon regulated myeloid specific secreted cysteine rich protein C/EBP epsilon regulated myeloid specific secreted cysteine rich protein precursor 1 C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein CG5403 Cysteine rich secreted protein A12 alpha like 2 Cysteine rich secreted protein FIZZ3 Cysteine-rich secreted protein A12-alpha-like 2 Cysteine-rich secreted protein FIZZ3 dri FIZZ 3 FIZZ3 Found in inflammatory zone 3 HXCP 1 HXCP1 MGC126603 MGC126609 PRO1199 Resistin Resistin delta2 RETN 1 RETN RETN1 RSTN UNQ407 XCP 1 XCP1 |
Images
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Fig1:
Sandwich ELISA analysis of Mouse Resistin matched pair antibodies Capture: HA723808, Mouse Resistin Rabbit mAb [PSH16-03] Detector: HA723809, Mouse Resistin Rabbit mAb [PSH16-04] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723808) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Resistin protein (HA211042) starting from 100,000 pg/ml to 0 pg/ml and detect antibody (HA723809, HRP, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.