Alpha 1 antitrypsin Recombinant Rabbit Monoclonal Antibody [PSH16-21]
cat.: HA723819
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH16-21 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 46 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse Alpha 1 antitrypsin aa 25-413. |
| Positive control: | Mouse liver tissue lysate, Mouse heart tissue lysate, Mouse kidney tissue lysate, Rat spleen ver tissue lysate, mouse liver tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P |
1:50,000 1:10,000 |
| Uniprot #: | SwissProt: P07758 Mouse | P17475 Rat |
| Alternative names: | A1A A1AT A1AT_HUMAN AAT Alpha 1 antiproteinase Alpha 1 antitrypsin Alpha 1 antitrypsin null Alpha 1 protease inhibitor Alpha-1 protease inhibitor Alpha-1-antiproteinase alpha1 proteinase inhibitor Alpha1AT Dom1 PI PI1 PRO0684 PRO2275 Serine (or cysteine) proteinase inhibitor clade A member 1 Serine protease inhibitor 1-1 Serine protease inhibitor A1a Serpin A1 Serpin A1a Serpin peptidase inhibitor clade A member 1 Serpina1 Short peptide from AAT SPAAT Spi1-1 |
Images
|
Fig1:
Western blot analysis of Alpha 1 antitrypsin on different lysates with Rabbit anti-Alpha 1 antitrypsin antibody (HA723819) at 1/50,000 dilution. Lane 1: Mouse liver tissue lysate (20 µg/Lane) Lane 2: Mouse heart tissue lysate (20 µg/Lane) Lane 3: Mouse kidney tissue lysate (20 µg/Lane) Lane 4: Rat spleen ver tissue lysate (40 µg/Lane) Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723819) at 1/50,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Alpha 1 antitrypsin antibody (HA723819) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723819) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.