SCNN1G Recombinant Rabbit Monoclonal Antibody [PSH16-29]
cat.: HA723827
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Green monkey |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | PSH16-29 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 74 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human SCNN1G aa 77-541. |
| Positive control: | A549 cell lysate, A431 cell lysate, 293T cell lysate, COS-1 cell lysate, C2C12 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, C6 cell lysate, Mouse kidney tissue lysate, Mouse lung tissue lysate, Mouse colon tissue lysate, Rat kidney tissue lysate, Rat lung tissue lysate, Rat colon tissue lysate, 293T, PC-12. |
| Subcellular location: | Apical cell membrane. |
| Recommended Dilutions:
WB IF-Cell |
1:5,000 1:100-1:500 |
| Uniprot #: | SwissProt: P51170 Human | Q9WU39 Mouse | P37091 Rat |
| Alternative names: | Epithelial sodium channel subunit gamma ENaC subunit gamma ENaCG; Epithelial Na(+) channel subunit gamma; Gamma-ENaC Amiloride-sensitive sodium channel subunit gamma Gamma-NaCH Nonvoltage-gated sodium channel 1 subunit gamma SCNEG Sodium channel epithelial 1 subunit gamma SCNN1G |
Images
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Fig1:
Western blot analysis of SCNN1G on different lysates with Rabbit anti-SCNN1G antibody (HA723827) at 1/5,000 dilution. Lane 1: A549 cell lysate (20 µg/Lane) Lane 2: A431 cell lysate (20 µg/Lane) Lane 3: 293T cell lysate (20 µg/Lane) Lane 4: COS-1 cell lysate (20 µg/Lane) Lane 5: C2C12 cell lysate (20 µg/Lane) Lane 6: NIH/3T3 cell lysate (20 µg/Lane) Lane 7: PC-12 cell lysate (20 µg/Lane) Lane 8: C6 cell lysate (20 µg/Lane) Lane 9: Mouse kidney tissue lysate (40 µg/Lane) Lane 10: Mouse lung tissue lysate (40 µg/Lane) Lane 11: Mouse colon tissue lysate (40 µg/Lane) Lane 12: Rat kidney tissue lysate (40 µg/Lane) Lane 13: Rat lung tissue lysate (40 µg/Lane) Lane 14: Rat colon tissue lysate (40 µg/Lane) Predicted band size: 74 kDa Observed band size: 74 kDa Exposure time: 16 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723827) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of 293T cells labeling SCNN1G with Rabbit anti-SCNN1G antibody (HA723827) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SCNN1G antibody (HA723827) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunocytochemistry analysis of PC-12 cells labeling SCNN1G with Rabbit anti-SCNN1G antibody (HA723827) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SCNN1G antibody (HA723827) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.