Biotin Conjugated Mouse VCAM1 Recombinant Rabbit Monoclonal Antibody [PSH16-32]
cat.: HA723830B
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Mouse
Applications: ELISA(Det), ELISA
Clonality: Monoclonal
Clone number: PSH16-32
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Isotype: IgG
Immunogen: Recombinant protein within Mouse VCAM1 aa 25-698 (HA211043).
Positive control: Recombinant Mouse VCAM1 protein (HA211043).
Subcellular location: Cell membrane; Secreted.
Recommended Dilutions:
  ELISA(Det)
  ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH16-31] to Mouse VCAM1 antibody (Capture) (HA723829) or Rabbit monoclonal [PSH16-33] to Mouse VCAM1 antibody (Capture) (HA723831) and Recombinant Mouse VCAM1 protein (HA211043) as the standard. The reference range value is 31.3-8,000 pg/mL.
Use at an assay dependent concentration.
Uniprot #: SwissProt: P29533 Mouse
Alternative names: CD106 CD106 Antigen INCAM 100 INCAM-100 L1CAM MGC99561 V-CAM 1 Vascular Cell Adhesion Molecule 1 Vascular cell adhesion protein 1 VCAM 1 VCAM-1 VCAM1
Images
HA723830B_1.png Fig1: Sandwich ELISA analysis of Mouse VCAM1 matched pair antibodies

Capture: HA723829, Mouse VCAM1 Rabbit mAb [PSH16-31]
Detector: HA723830, Mouse VCAM1 Rabbit mAb [PSH16-32]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723829) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse VCAM1 protein (HA211043) starting from 8,000 pg/ml to 0 pg/ml and detect antibody (HA723830, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
HA723830B_2.png Fig2: Sandwich ELISA analysis of Mouse VCAM1 matched pair antibodies

Capture: HA723831, Mouse VCAM1 Rabbit mAb [PSH16-33]
Detector: HA723830, Mouse VCAM1 Rabbit mAb [PSH16-32]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723831) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse VCAM1 protein (HA211043) starting from 8,000 pg/ml to 0 pg/ml and detect antibody (HA723830, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
HA723830B_3.png Fig3: Interpolated concentrations of native VCAM1 in Mouse serum and
Raw264.7 cell culture supernatantl.

Capture: HA723829, Mouse VCAM1 Rabbit mAb [PSH16-31]
Detector: HA723830, Mouse VCAM1 Rabbit mAb [PSH16-32]

Interpolated concentration of native VCAM1 was measured in duplicate at different sample concentrations and interpolated from the VCAM1 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean VCAM1 concentration was determined to be 3,001,110 pg/ml in Mouse serum and undetectable in Raw264.7 cell culture supernatant.
HA723830B_4.png Fig4: Interpolated concentrations of native VCAM1 in Mouse serum and
Raw264.7 cell culture supernatantl.

Capture: HA723831, Mouse VCAM1 Rabbit mAb [PSH16-33]
Detector: HA723830, Mouse VCAM1 Rabbit mAb [PSH16-32]

Interpolated concentration of native VCAM1 was measured in duplicate at different sample concentrations and interpolated from the VCAM1 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean VCAM1 concentration was determined to be 3,225,681 pg/ml in Mouse serum and undetectable in Raw264.7 cell culture supernatant.
HA723830B_5.png Fig5: Interpolated concentrations of spiked VCAM1 in cell culture media samples.

Capture: HA723829, Mouse VCAM1 Rabbit mAb [PSH16-31]
Detector: HA723830, Mouse VCAM1 Rabbit mAb [PSH16-32]

The concentrations of VCAM1 were measured in duplicates, interpolated from the VCAM1 standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
HA723830B_6.png Fig6: Interpolated concentrations of spiked VCAM1 in cell culture media samples.

Capture: HA723831, Mouse VCAM1 Rabbit mAb [PSH16-33]
Detector: HA723830, Mouse VCAM1 Rabbit mAb [PSH16-32]

The concentrations of VCAM1 were measured in duplicates, interpolated from the VCAM1 standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.