HRP Conjugated Mouse VCAM1 Recombinant Rabbit Monoclonal Antibody [PSH16-32]
cat.: HA723830H
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH16-32 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse VCAM1 aa 25-698 (HA211043). |
| Positive control: | Recombinant Mouse VCAM1 protein (HA211043). |
| Subcellular location: | Cell membrane; Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH16-31] to Mouse VCAM1 antibody (Capture) (HA723829) or Rabbit monoclonal [PSH16-33] to Mouse VCAM1 antibody (Capture) (HA723831) and Recombinant Mouse VCAM1 protein (HA211043) as the standard. The reference range value is 625-20,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P29533 Mouse |
| Alternative names: | CD106 CD106 Antigen INCAM 100 INCAM-100 L1CAM MGC99561 V-CAM 1 Vascular Cell Adhesion Molecule 1 Vascular cell adhesion protein 1 VCAM 1 VCAM-1 VCAM1 |
Images
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Fig1:
Sandwich ELISA analysis of Mouse VCAM1 matched pair antibodies Capture: HA723829, Mouse VCAM1 Rabbit mAb [PSH16-31] Detector: HA723830, Mouse VCAM1 Rabbit mAb [PSH16-32] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723829) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse VCAM1 protein (HA211043) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA723830, HRP, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.