HRP Conjugated Mouse ICAM1 Recombinant Rabbit Monoclonal Antibody [PSH16-35]
cat.: HA723833H
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH16-35 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse ICAM1 aa 24-485 (HA211066). |
| Positive control: | Recombinant Mouse ICAM1 protein (HA211066). |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH16-34] to Mouse ICAM1 antibody (Capture) (HA723832) or Rabbit monoclonal [PSH16-36] to Mouse ICAM1 antibody (Capture) (HA723834) and Recombinant Mouse ICAM1 protein (HA211066) as the standard. The reference range value is 312.5-40,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P13597 Mouse |
| Alternative names: | Antigen identified by monoclonal BB2 BB 2 BB2 CD 54 CD_antigen=CD54 CD54 Cell surface glycoprotein P3.58 Human rhinovirus receptor ICAM 1 ICAM-1 ICAM1 intercellular adhesion molecule 1 (CD54) intercellular adhesion molecule 1 (CD54), human rhinovirus receptor Intercellular adhesion molecule 1 Major group rhinovirus receptor MALA 2 MALA2 MyD 10 MyD10 P3.58 Surface antigen of activated B cells Surface antigen of activated B cells, BB2 |
Images
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Fig1:
Sandwich ELISA analysis of Mouse ICAM1 matched pair antibodies Capture: HA723834, Mouse ICAM1 Rabbit mAb [PSH16-36] Detector: HA723833, Mouse ICAM1 Rabbit mAb [PSH16-35] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723834) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse ICAM1 protein (HA211066) starting from 40,000 pg/ml to 0 pg/ml and detect antibody (HA723833, HRP, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.