Human Fas / CD95 Recombinant Rabbit Monoclonal Antibody [PSH16-39]
cat.: HA723837
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH16-39 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Fas / CD95 aa 26-173 (HA211064). |
| Positive control: | Recombinant Human Fas / CD95 protein (HA211064). |
| Subcellular location: | Cell membrane, Membrane raft; Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH16-38] to Human Fas / CD95 antibody (Detector) (HA723836) and Recombinant Human Fas / CD95 protein (HA211064) as the standard. The reference range value is 15.6-4,000 pg/mL. |
| Uniprot #: | SwissProt: P25445 Human |
| Alternative names: | ALPS 1A ALPS1A APO 1 Apo 1 antigen APO 1 cell surface antigen Apo-1 antigen APO1 Apo1 antigen APO1 cell surface antigen Apoptosis antigen 1 Apoptosis mediating surface antigen FAS Apoptosis-mediating surface antigen FAS APT 1 APT1 CD 95 CD 95 antigen CD95 CD95 antigen Delta Fas Delta Fas/APO 1/CD95 Delta Fas/APO1/CD95 Fas (TNF receptor superfamily, member 6) FAS 1 FAS 827dupA Fas AMA Fas FAS Antigen Fas cell surface death receptor FAS1 FASLG receptor FASTM sFAS Surface antigen APO1 TNF receptor superfamily, member 6 TNFRSF 6 TNFRSF6 TNR6_HUMAN Tumor necrosis factor receptor superfamily member 6 |
Images
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Fig1:
Sandwich ELISA analysis of Human Fas / CD95 matched pair antibodies Capture: HA723837, Human Fas/CD95 Rabbit mAb [PSH16-39] Detector: HA723836, Human Fas/CD95 Rabbit mAb [PSH16-38] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723837) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Fas / CD95 protein (HA211064) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723836, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native FAS in Raji，Ramos and K-562 Cell extract samples based on a 1000 µg/ml extract load. Capture: HA723837, Human Fas/CD95 Rabbit mAb [PSH16-39] Detector: HA723836, Human Fas/CD95 Rabbit mAb [PSH16-38] Interpolated concentration of native FAS was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean FAS concentration was determined to be 15,671 pg/mL in Raji Cell extract 1,643 pg/mL in Ramos Cell extract and undetectable in K-562 Cell extract. |
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Fig3:
Interpolated concentrations of spiked FAS in cell culture media samples. Capture: HA723837, Human Fas/CD95 Rabbit mAb [PSH16-39] Detector: HA723836, Human Fas/CD95 Rabbit mAb [PSH16-38] The concentrations of FAS were measured in duplicates, interpolated from the FAS standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.