Brain-derived tau/BD-tau Recombinant Rabbit Monoclonal Antibody [PSH16-47]
cat.: HA723848
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH16-47 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Tau aa 5-24 (P10636-8). |
| Positive control: | Human Tau/Tau441, C-His (isoform 2N4R) Protein (HA210937). |
| Subcellular location: | Cell membrane. Cytoplasm. Secreted. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH16-46] to Brain-derived tau/BD-tau antibody (Capture) (HA723847) and Recombinant Human Tau/Tau441 protein (HA210937) as the standard. The reference range value is 0.156-10 ng/mL. |
| Uniprot #: | SwissProt: P10636-8 Human |
| Alternative names: | AI413597 AW045860 DDPAC FLJ31424 FTDP 17 G protein beta1/gamma2 subunit interacting factor 1 MAPT MAPTL MGC134287 MGC138549 MGC156663 Microtubule associated protein tau Microtubule associated protein tau isoform 4 Microtubule-associated protein tau MSTD Mtapt MTBT1 MTBT2 Neurofibrillary tangle protein Paired helical filament tau Paired helical filament-tau PHF tau PHF-tau PPND PPP1R103 Protein phosphatase 1, regulatory subunit 103 pTau RNPTAU TAU TAU_HUMAN Tauopathy and respiratory failure Tauopathy and respiratory failure, included |
Images
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Fig1:
Sandwich ELISA analysis of Human Brain-derived tau/BD-tau matched pair antibodies Capture: HA723847, Brain-derived tau/BD-tau Rabbit mAb [PSH16-46] Detector: HA723848, Brain-derived tau/BD-tau Rabbit mAb [PSH10-47] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723847) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Brain-derived tau/BD-tau protein (HA210937) starting from 10000 pg/ml to 0 pg/ml and detect antibody (HA723848, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native Brain-derived Tau in SH-SY5Y and MDA-MB-231 cell extract samples based on a 1000 µg/ml extract load. Capture: HA723847, Brain-derived tau/BD-tau Rabbit mAb [PSH16-46] Detector: HA723848, Brain-derived tau/BD-tau Rabbit mAb [PSH10-47] Interpolated concentration of native Brain-derived Tau was measured in duplicate at different sample concentrations and interpolated from the Brain-derived Tau standard curves. The mean Brain-derived Tau concentration was determined to be 40,370 pg/mL in SH-SY5Y cell extract. There was no detectable signal in MDA-MB-231 cell extract. |
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Fig3:
Interpolated concentrations of spiked Brain-derived Tau in cell culture media samples. Capture: HA723847, Brain-derived tau/BD-tau Rabbit mAb [PSH16-46] Detector: HA723848, Brain-derived tau/BD-tau Rabbit mAb [PSH10-47] The concentrations of Brain-derived Tau were measured in duplicates, interpolated from the Brain-derived Tau standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
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Fig4:
Coating Ab: HA723847, 5ug/ml Co-Incubation: 1:2 serially diluted human Brain-derived Tau protein and Big Tau from HuaBio starting from a concentration of 10,000 pg/ml and Detect Ab: HA723848, Biotin, 0.2 μg/ml (30℃, 120rpm, 60min). SA-HRP: 1:20,000 (50ng/ml) (30℃, 120rpm, 30min). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.