Tau Recombinant Rabbit Monoclonal Antibody [PSH16-48]
cat.: HA723850
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH16-48 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within HumanTau aa 5-24. |
| Positive control: | Human Tau/Tau441, C-His (isoform 2N4R) Protein (HA210937). |
| Subcellular location: | Cell membrane. Cytoplasm. Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH10-42] to Tau antibody (Detector) (HA723209) or Rabbit monoclonal [PSH16-49] to Tau antibody (Detector) (HA723851) and Recombinant Human Tau protein (HA210937) as the standard. The reference range value is 39-5,000 pg/mL. |
| Uniprot #: | SwissProt: P10636 Human | P10637 Mouse | P19332 Rat |
| Alternative names: | AI413597 AW045860 DDPAC FLJ31424 FTDP 17 G protein beta1/gamma2 subunit interacting factor 1 MAPT MAPTL MGC134287 MGC138549 MGC156663 Microtubule associated protein tau Microtubule associated protein tau isoform 4 Microtubule-associated protein tau MSTD Mtapt MTBT1 MTBT2 Neurofibrillary tangle protein Paired helical filament tau Paired helical filament-tau PHF tau PHF-tau PPND PPP1R103 Protein phosphatase 1, regulatory subunit 103 pTau RNPTAU TAU TAU_HUMAN Tauopathy and respiratory failure Tauopathy and respiratory failure, included |
Images
|
Fig1:
Sandwich ELISA analysis of human Tau matched pair antibodies Capture: HA723850, Tau Rabbit mAb [PSH16-48] Detector: HA723209, Tau Rabbit mAb [PSH10-42] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723850) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Tau protein (HA210937) starting from 10000 pg/ml to 0 pg/ml and detect antibody (HA723209, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Sandwich ELISA analysis of human Tau matched pair antibodies Capture: HA723850, Tau Rabbit mAb [PSH16-48] Detector: HA723209, Tau Rabbit mAb [PSH10-42] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723850) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Tau protein (HA211246) starting from 10000 pg/ml to 0 pg/ml and detect antibody (HA723209, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig3:
Interpolated concentrations of native Tau in SH-SY5Y and mouse cerebellum extract samples based on a 1000 µg/ml extract load. Capture: HA723850, Tau Rabbit mAb [PSH16-48] Detector: HA723209, Tau Rabbit mAb [PSH10-42] Interpolated concentration of native Tau was measured in duplicate at different sample concentrations and interpolated from the Tau standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Tau concentration was determined to be 14,861 pg/mL in SH-SY5Y cell extract and 3,257 pg/mL in mouse cerebellum tissue extract. |
|
Fig4:
Interpolated concentrations of spiked Tau in cell culture media samples. Capture: HA723850, Tau Rabbit mAb [PSH16-48] Detector: HA723209, Tau Rabbit mAb [PSH10-42] The concentrations of Tau were measured in duplicates, interpolated from the Tau standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
|
Fig5:
Sandwich ELISA analysis of human Tau matched pair antibodies Capture: HA723850, Tau Rabbit mAb [PSH16-48] Detector: HA723851, Tau Rabbit mAb [PSH16-49] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723850) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Tau protein (HA210937) starting from 10000 pg/ml to 0 pg/ml and detect antibody (HA723851, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig6:
Interpolated concentrations of native Tau (4R Isoforms) in SH-SY5Y extract samples based on a 1000 µg/ml extract load. Capture: HA723850, Tau Rabbit mAb [PSH16-48] Detector: HA723851, Tau Rabbit mAb [PSH16-49] Interpolated concentration of native Tau (4R Isoforms) was measured in duplicate at different sample concentrations and interpolated from the Tau (4R Isoforms) standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Tau (4R Isoforms) concentration was determined to be 41,927 pg/mL in SH-SY5Y cell extract. |
|
Fig7:
Interpolated concentrations of native Tau (4R Isoforms) in human cell culture supernatant samples. Capture: HA723850, Tau Rabbit mAb [PSH16-48] Detector: HA723851, Tau Rabbit mAb [PSH16-49] Interpolated concentration of native Tau (4R Isoforms) was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Tau (4R Isoforms) concentration was determined to be 2,372 pg/mL in SH-SY5Y cell supernatant. There was no detectable signal in MDA-MB-231 cell culture supernatant. |
|
Fig8:
Interpolated concentrations of spiked Tau (4R Isoforms) in cell culture media samples. Capture: HA723850, Tau Rabbit mAb [PSH16-48] Detector: HA723851, Tau Rabbit mAb [PSH16-49] The concentrations of Tau (4R Isoforms) were measured in duplicates, interpolated from the Tau (4R Isoforms) standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.