HA723859

TAP2 Recombinant Rabbit Monoclonal Antibody [PSH16-55]

cat.: HA723859

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IHC-P, IP
Clonality:	Monoclonal
Clone number:	PSH16-55

Form:	Liquid
Storage condition:	Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer:	1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 76 kDa
Isotype:	IgG
Immunogen:	Recombinant protein within human TAP2 aa 420-686.
Positive control:	HeLa cell lysate, Jurkat cell lysate, Raji cell lysate, MoLT-4 cell lysate, SW480 cell lysate, HeLa treated with 100ng/mL IFN gamma for 72 hours cell lysate, human tonsil tissue, human spleen tissue, mouse spleen tissue, rat spleen tissue.
Subcellular location:	Endoplasmic reticulum membrane.
Recommended Dilutions: WB IHC-P IP	1:5,000-1:20,000 1:200-1:1,000 1-2μg/sample
Uniprot #:	SwissProt: Q03519 Human \| P36371 Mouse \| P36372 Rat
Alternative names:	ABC transporter, MHC 2 ABC18 ABCB3 Antigen peptide transporter 2 APT2 ATP binding cassette, sub family B (MDR/TAP), member 3 ATP-binding cassette sub-family B member 3 D6S217E Peptide supply factor 2 Peptide transporter involved in antigen processing 2 Peptide transporter PSF2 Peptide transporter TAP2 PSF 2 PSF-2 PSF2 Really interesting new gene 11 protein RING 11 RING11 TAP 2 Tap2 TAP2_HUMAN Transporter 2 ATP binding cassette sub family B Transporter 2, ABC (ATP binding cassette Transporter 2, ATP binding cassette, sub family B (MDR/TAP)

Images

Fig1: Western blot analysis of TAP2 on different lysates with Rabbit anti-TAP2 antibody (HA723859) at 1/20,000 dilution.

Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: Jurkat cell lysate (20 µg/Lane)
Lane 3: Raji cell lysate (20 µg/Lane)
Lane 4: MoLT-4 cell lysate (20 µg/Lane)
Lane 5: SW480 cell lysate (20 µg/Lane)

Predicted band size: 76 kDa
Observed band size: 76 kDa

Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723859) at 1/20,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Fig2: Western blot analysis of TAP2 on different lysates with Rabbit anti-TAP2 antibody (HA723859) at 1/20,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 100ng/mL IFN gamma for 72 hours cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 76 kDa
Observed band size: 76 kDa

Exposure time: 4 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723859) at 1/20,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

	Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-TAP2 antibody (HA723859) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA723859) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-TAP2 antibody (HA723859) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA723859) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-TAP2 antibody (HA723859) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA723859) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig6: Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-TAP2 antibody (HA723859) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA723859) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig7: TAP2 was immunoprecipitated from 0.2 mg MOLT-4 cell lysate with HA723859 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723859 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

Lane 1: MOLT-4 cell lysate (input)
Lane 2: HA723859 IP in MOLT-4 cell lysate
Lane 3: Rabbit IgG instead of HA723859 in MOLT-4 cell lysate

Blocking/Dilution buffer: primary antibody dilution (K1803)
Exposure time: 10 seconds; ECL: K1801

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.