Biotin Conjugated Human RANKL Recombinant Rabbit Monoclonal Antibody [PSH16-59]
cat.: HA723861B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH16-59 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human RANKL aa 140-317 (HA211170). |
| Positive control: | Recombinant Human RANKL protein (HA211170). |
| Subcellular location: | Cell membrane, Cytoplasm, Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH16-58] to Human RANKL antibody (Capture) (HA723860) or Rabbit monoclonal [PSH16-60] to Human RANKL antibody (Capture) (HA723862) and Recombinant Human RANKL protein (HA211170) as the standard. The reference range value is 15.6-2,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: O14788 Human |
| Alternative names: | CD254 hRANKL2 ODF OPGL OPTB2 Osteoclast differentiation factor Osteoprotegerin ligand Rank Ligand RANKL Receptor activator of nuclear factor kappa B ligand Receptor activator of nuclear factor kappa-B ligand sOdf TNF related activation induced cytokine TNF-related activation-induced cytokine TNF11_HUMAN TNFSF 11 Tnfsf11 TRANCE Tumor necrosis factor (ligand) superfamily member 11 Tumor necrosis factor ligand superfamily member 11 Tumor necrosis factor ligand superfamily member 11, soluble form |
Images
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Fig1:
Sandwich ELISA analysis of Human RANKL matched pair antibodies Capture: HA723860, Human RANKL Rabbit mAb [PSH16-58] Detector: HA723861, Human RANKL Rabbit mAb [PSH16-59] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723860) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human RANKL protein (HA211170) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723861, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Sandwich ELISA analysis of Human RANKL matched pair antibodies Capture: HA723862, Human RANKL Rabbit mAb [PSH16-60] Detector: HA723861, Human RANKL Rabbit mAb [PSH16-59] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723862) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human RANKL protein (HA211170) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723861, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig3:
Interpolated concentrations of spiked TNFSF11 in human cell culture media samples. Capture: HA723860, Human RANKL Rabbit mAb [PSH16-58] Detector: HA723861, Human RANKL Rabbit mAb [PSH16-59] The concentrations of TNFSF11 were measured in duplicates, interpolated from the TNFSF11 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
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Fig4:
Interpolated concentrations of spiked TNFSF11 in human cell culture media samples. Capture: HA723862, Human RANKL Rabbit mAb [PSH16-60] Detector: HA723861, Human RANKL Rabbit mAb [PSH16-59] The concentrations of TNFSF11 were measured in duplicates, interpolated from the TNFSF11 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.