Mouse IL-33 Recombinant Rabbit Monoclonal Antibody [PSH16-62]
cat.: HA723864
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Mouse
Applications: ELISA(Det)
Clonality: Monoclonal
Clone number: PSH16-62
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4).
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Isotype: IgG
Immunogen: Recombinant protein within Mouse IL-33 aa 109-266 (HA210768).
Positive control: Recombinant Mouse IL-33 protein (HA210768).
Subcellular location: Nucleus, Chromosome, Cytoplasm, Cytoplasmic vesicle, secretory vesicle, Secreted.
Recommended Dilutions:
  ELISA(Det)
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH16-61] to Mouse IL-33 antibody (Capture) (HA723863) or Rabbit monoclonal [PSH16-63] to Mouse IL-33 antibody (Capture) (HA723865) and Recombinant Mouse IL-33 protein (HA210768) as the standard. The reference range value is 7.8-2,000 pg/mL.
Uniprot #: SwissProt: Q8BVZ5 Mouse
Alternative names: C9orf26 CHROMOSOME 9 OPEN READING FRAME 26 DKFZp586H0523 DVS27 DVS27 related protein IL 1F11 IL 33 IL-1F11 IL-33 IL1F11 IL33 IL33_HUMAN Interleukin 1 family member 11 Interleukin 33 INTERLEUKIN 33 NFHEV Interleukin 33 precursor Interleukin-1 family member 11 Interleukin-33 (109-270) Interleukin33 NF HEV NF-HEV NFEHEV NFHEV Nuclear factor for high endothelial venules Nuclear factor from high endothelial venules OTTHUMP00000021041 RP11 575C20.2
Images
HA723864_1.png Fig1: Sandwich ELISA analysis of Mouse IL-33 matched pair antibodies

Capture: HA723863, Mouse IL-33 Rabbit mAb [PSH16-61]
Detector: HA723864, Mouse IL-33 Rabbit mAb [PSH16-62]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723863) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-33 protein (HA210768) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723864, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
HA723864_2.png Fig2: Sandwich ELISA analysis of Mouse IL-33 matched pair antibodies

Capture: HA723865, Mouse IL-33 Rabbit mAb [PSH16-63]
Detector: HA723864, Mouse IL-33 Rabbit mAb [PSH16-62]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723865) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-33 protein (HA210768) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723864, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
HA723864_3.png Fig3: Interpolated concentrations of native IL-33 in Mouse lung supernatant.

Capture: HA723863, Mouse IL-33 Rabbit mAb [PSH16-61]
Detector: HA723864, Mouse IL-33 Rabbit mAb [PSH16-62]

Interpolated concentration of native IL-33 was measured in duplicate at different sample concentrations and interpolated from the IL-33 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean IL-33 concentration was determined to be 113 pg/ml in Mouse lung supernatant.
HA723864_4.png Fig4: Interpolated concentrations of native IL-33 in Mouse lung supernatant .

Capture: HA723865, Mouse IL-33 Rabbit mAb [PSH16-63]
Detector: HA723864, Mouse IL-33 Rabbit mAb [PSH16-62]

Interpolated concentration of native IL-33 was measured in duplicate at different sample concentrations and interpolated from the IL-33 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean IL-33 concentration was determined to be 160 pg/ml in Mouse lung supernatant.
HA723864_5.png Fig5: Interpolated concentrations of spiked IL-33 in cell culture media samples.

Capture: HA723863, Mouse IL-33 Rabbit mAb [PSH16-61]
Detector: HA723864, Mouse IL-33 Rabbit mAb [PSH16-62]

The concentrations of IL-33 were measured in duplicates, interpolated from the IL-33 standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
HA723864_6.png Fig6: Interpolated concentrations of spiked IL-33 in cell culture media samples.

Capture: HA723865, Mouse IL-33 Rabbit mAb [PSH16-63]
Detector: HA723864, Mouse IL-33 Rabbit mAb [PSH16-62]

The concentrations of IL-33 were measured in duplicates, interpolated from the IL-33 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.