Biotin Conjugated Mouse/Rat GDF15 Recombinant Rabbit Monoclonal Antibody [PSH16-65]
cat.: HA723867B
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Mouse, Rat
Applications: ELISA(Det), ELISA
Clonality: Monoclonal
Clone number: PSH16-65
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Isotype: IgG
Immunogen: Recombinant protein within Mouse GDF-15 aa 189-303 (HA211089).
Positive control: Recombinant Mouse GDF15 protein (HA211089).
Subcellular location: Secreted.
Recommended Dilutions:
  ELISA(Det)
  ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH16-64] to Mouse GDF15 antibody (Capture) (HA723866) or Rabbit monoclonal [PSH16-66] to Mouse GDF15 antibody (Capture) (HA723868) and Recombinant Mouse GDF15 protein (HA211089) as the standard. The reference range value is 7.8-1,000 pg/mL.
Use at an assay dependent concentration.
Uniprot #: SwissProt: Q9Z0J7 Mouse | Q9Z0J6 Rat
Alternative names: GDF 15 GDF-15 Gdf15 GDF15_HUMAN Growth differentiation factor 15 Growth/differentiation factor 15 Macrophage inhibitory cytokine 1 MIC 1 Mic-1 MIC1 NAG 1 NAG-1 NAG1 NRG 1 NRG-1 NRG1 NSAID (nonsteroidal anti inflammatory drug) activated protein 1 NSAID NSAID regulated protein 1 NSAID-activated gene 1 protein NSAID-regulated gene 1 protein PDF PLAB Placental bone morphogenetic protein Placental bone morphogenic protein Placental TGF beta Placental TGF-beta Prostate differentiation factor PTGF beta PTGFB
Images
HA723867B_1.png Fig1: Sandwich ELISA analysis of Mouse GDF15 matched pair antibodies

Capture: HA723866, Mouse GDF15 Rabbit mAb [PSH16-64]
Detector: HA723867, Mouse GDF15 Rabbit mAb [PSH16-65]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723866) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse GDF15 protein (HA211089) protein starting from 1000 pg/ml to 0 pg/ml and detect antibody (HA723867, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
HA723867B_2.png Fig2: Sandwich ELISA analysis of Mouse GDF15 matched pair antibodies

Capture: HA723868, Mouse GDF15 Rabbit mAb [PSH16-66]
Detector: HA723867, Mouse GDF15 Rabbit mAb [PSH16-65]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723868) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse GDF15 protein (HA211089) protein starting from 1000 pg/ml to 0 pg/ml and detect antibody (HA723867, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
HA723867B_3.png Fig3: Interpolated concentrations of native GDF-15 in mouse and rat samples.

Capture: HA723866, Mouse GDF15 Rabbit mAb [PSH16-64]
Detector: HA723867, Mouse GDF15 Rabbit mAb [PSH16-65]

Interpolated concentration of native GDF-15 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean GDF-15 concentration was determined to be 21,738 pg/mL in Hepa1-6 cell culture supernatant and 269 pg/mL in rat serum.
HA723867B_4.png Fig4: Interpolated concentrations of native GDF-15 in mouse and
rat samples.

Capture: HA723868, Mouse GDF15 Rabbit mAb [PSH16-66]
Detector: HA723867, Mouse GDF15 Rabbit mAb [PSH16-65]

Interpolated concentration of native GDF-15 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean GDF-15 concentration was determined to be 21,079 pg/mL in Hepa1-6 cell culture supernatant and 449 pg/mL in rat serum.
HA723867B_5.png Fig5: Interpolated concentrations of spiked GDF-15 in cell culture media samples.

Capture: HA723866, Mouse GDF15 Rabbit mAb [PSH16-64]
Detector: HA723867, Mouse GDF15 Rabbit mAb [PSH16-65]

The concentrations of GDF-15 were measured in duplicates, interpolated from the GDF-15 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
HA723867B_6.png Fig6: Interpolated concentrations of spiked GDF-15 in cell culture media samples.

Capture: HA723868, Mouse GDF15 Rabbit mAb [PSH16-66]
Detector: HA723867, Mouse GDF15 Rabbit mAb [PSH16-65]

The concentrations of GDF-15 were measured in duplicates, interpolated from the GDF-15 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.