HRP Conjugated P-Selectin / CD62P Recombinant Rabbit Monoclonal Antibody [PSH16-71]
cat.: HA723873H
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH16-71 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse P-Selectin / CD62P aa 42-709 (HA211140). |
| Positive control: | Recombinant Human P-Selectin / CD62P protein (HA210931) or Recombinant Mouse P-Selectin / CD62P protein (HA211140). |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH16-71] to P-Selectin / CD62P antibody (Detector) (HA723873) and Recombinant Human P-Selectin / CD62P protein (HA210931) or Recombinant Mouse P-Selectin / CD62P protein (HA211140) as the standard. The reference range value is 1,370-111,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P16109 Human | Q01102 Mouse | P98106 Rat |
| Alternative names: | Antigen CD62 CD62 CD62 antigen-like family member P CD62P GMP 140 GMP-140 GMP140 GMRP Granule membrane protein 140 Granulocyte membrane protein GRMP LECAM 3 LECAM3 Leukocyte endothelial cell adhesion molecule 3 Leukocyte-endothelial cell adhesion molecule 3 LYAM3_HUMAN P Selectin P-selectin PADGEM Platelet activation dependent granule-external membrane protein Platelet alpha granule membrane protein PSEL Selectin P (granule membrane protein 140kDa, antigen CD62) SELP |
Images
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Fig1:
Sandwich ELISA analysis of Human P-Selectin matched pair antibodies Capture: HA723872, P-Selectin / CD62P Rabbit mAb [PSH16-70] Detector: HA723873, P-Selectin / CD62P Rabbit mAb [PSH16-71] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723872) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human P-Selectin / CD62P protein (HA210931) starting from 111,000 pg/ml to 0 pg/ml and detect antibody (HA723873, HRP, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.