HA723875B

Biotin Conjugated Mouse L-Selectin / CD62L Recombinant Rabbit Monoclonal Antibody [PSH16-73]

cat.: HA723875B

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Mouse
Applications:	ELISA(Det), ELISA
Clonality:	Monoclonal
Clone number:	PSH16-73

Form:	Liquid
Storage condition:	Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer:	1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Isotype:	IgG
Immunogen:	Recombinant protein within Mouse L-Selectin / CD62L aa 39-332 (HA211126).
Positive control:	Recombinant Mouse L-Selectin / CD62L protein (HA211126).
Subcellular location:	Cell membrane.
Recommended Dilutions: ELISA(Det) ELISA	Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH16-72] to Mouse L-Selectin / CD62L antibody (Capture) (HA723874) or Rabbit monoclonal [PSH16-74] to Mouse L-Selectin / CD62L antibody (Capture) (HA723876) and Recombinant Mouse L-Selectin / CD62L protein (HA211126) as the standard. The reference range value is 15.6-4,000 pg/mL. Use at an assay dependent concentration.
Uniprot #:	SwissProt: P18337 Mouse
Alternative names:	A.11 AI528707 CD62 antigen ligand CD62 antigen-like family member L CD62L gp90-MEL IgA nephropathy, susceptibility to, included L Selectin L-selectin LAM-1 LAM1 LECAM1 LEU8 Leukocyte adhesion molecule 1 Leukocyte surface antigen Leu-8 Leukocyte-endothelial cell adhesion molecule 1 Lnhr LSEL Ly-22 Ly-m22 Lyam-1 LYAM1 LYAM1_HUMAN Lymph node homing receptor Lymphocyte adhesion molecule 1 Lymphocyte antigen 22 Lymphocyte surface MEL-14 antigen MEL-14 Pln homing receptor PLNHR Selectin L Selectin, lymphocyte SELL TQ1

Images

Fig1: Sandwich ELISA analysis of Mouse L-Selectin / CD62L matched pair antibodies

Capture: HA723874, Mouse L-Selectin / CD62L Rabbit mAb [PSH16-72]
Detector: HA723875, Mouse L-Selectin / CD62L Rabbit mAb [PSH16-73]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723874) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse L-Selectin / CD62L protein (HA211126) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723875, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Fig2: Sandwich ELISA analysis of Mouse L-Selectin / CD62L matched pair antibodies

Capture: HA723876, Mouse L-Selectin / CD62L Rabbit mAb [PSH16-74]
Detector: HA723875, Mouse L-Selectin / CD62L Rabbit mAb [PSH16-73]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723876) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse L-Selectin / CD62L protein (HA211126) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723875, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

	Fig3: Interpolated concentrations of native L-selectin in mouse serum . Capture: HA723874, Mouse L-Selectin / CD62L Rabbit mAb [PSH16-72] Detector: HA723875, Mouse L-Selectin / CD62L Rabbit mAb [PSH16-73] Interpolated concentration of native L-selectin was measured in duplicate at different sample concentrations and interpolated from the L-selectin standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean L-selectin concentration was determined to be 803,180 pg/ml in mouse serum.
	Fig4: Interpolated concentrations of native L-selectin in mouse serum . Capture: HA723876, Mouse L-Selectin / CD62L Rabbit mAb [PSH16-74] Detector: HA723875, Mouse L-Selectin / CD62L Rabbit mAb [PSH16-73] Interpolated concentration of native L-selectin was measured in duplicate at different sample concentrations and interpolated from the L-selectin standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean L-selectin concentration was determined to be 771,994 pg/ml in mouse serum.
	Fig5: Interpolated concentrations of spiked L-selectin in cell culture media samples. Capture: HA723874, Mouse L-Selectin / CD62L Rabbit mAb [PSH16-72] Detector: HA723875, Mouse L-Selectin / CD62L Rabbit mAb [PSH16-73] The concentrations of L-selectin were measured in duplicates, interpolated from the L-selectin standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Fig6: Interpolated concentrations of spiked L-selectin in cell culture media samples.

Capture: HA723876, Mouse L-Selectin / CD62L Rabbit mAb [PSH16-74]
Detector: HA723875, Mouse L-Selectin / CD62L Rabbit mAb [PSH16-73]

The concentrations of L-selectin were measured in duplicates, interpolated from the L-selectin standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.