HRP Conjugated Mouse L-Selectin / CD62L Recombinant Rabbit Monoclonal Antibody [PSH16-73]
cat.: HA723875H
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH16-73 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse L-Selectin / CD62L aa 39-332 (HA211126). |
| Positive control: | Recombinant Mouse L-Selectin / CD62L protein (HA211126). |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH16-72] to Mouse L-Selectin / CD62L antibody (Capture) (HA723874) or Rabbit monoclonal [PSH16-74] to Mouse L-Selectin / CD62L antibody (Capture) (HA723876) and Recombinant Mouse L-Selectin / CD62L protein (HA211126) as the standard. The reference range value is 1,370-111,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P18337 Mouse |
| Alternative names: | A.11 AI528707 CD62 antigen ligand CD62 antigen-like family member L CD62L gp90-MEL IgA nephropathy, susceptibility to, included L Selectin L-selectin LAM-1 LAM1 LECAM1 LEU8 Leukocyte adhesion molecule 1 Leukocyte surface antigen Leu-8 Leukocyte-endothelial cell adhesion molecule 1 Lnhr LSEL Ly-22 Ly-m22 Lyam-1 LYAM1 LYAM1_HUMAN Lymph node homing receptor Lymphocyte adhesion molecule 1 Lymphocyte antigen 22 Lymphocyte surface MEL-14 antigen MEL-14 Pln homing receptor PLNHR Selectin L Selectin, lymphocyte SELL TQ1 |
Images
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Fig1:
Sandwich ELISA analysis of Mouse L-Selectin / CD62L matched pair antibodies Capture: HA723874, Mouse L-Selectin / CD62L Rabbit mAb [PSH16-72] Detector: HA723875, Mouse L-Selectin / CD62L Rabbit mAb [PSH16-73] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723874) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse L-Selectin / CD62L protein (HA211126) starting from 111,000 pg/ml to 0 pg/ml and detect antibody (HA723875, HRP, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.