OTULIN Recombinant Rabbit Monoclonal Antibody [PSH16-95]
cat.: HA723891
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH16-95 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 40 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human OTULIN aa 1-352. |
| Positive control: | HeLa cell lysate, 786-0 cell lysate, U-2 OS. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB FC |
1:5,000 1:1,000 |
| Uniprot #: | SwissProt: Q96BN8 Human |
| Alternative names: | AIPDS Deubiquitinating enzyme otulin F105B_HUMAN Fam105b Family with sequence similarity 105, member B FLJ34884 GUM OTU deubiquitinase with linear linkage specificity OTULIN Protein FAM105B Ubiquitin thioesterase Gumby |
Images
|
Fig1:
Western blot analysis of OTULIN on different lysates with Rabbit anti-OTULIN antibody (HA723891) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: 786-0 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 40 kDa Observed band size: 42 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723891) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Flow cytometric analysis of U-2 OS cells labeling OTULIN. Cells were fixed and permeabilized. Then stained with the primary antibody (HA723891, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.