Mouse IL-17F Recombinant Rabbit Monoclonal Antibody [PSH17-15]
cat.: HA723902
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Det), ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH17-15 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse IL-17F aa 29-161 (HA211035). |
| Positive control: | Recombinant Mouse IL-17F protein (HA211035). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH17-14] to Mouse IL-17F antibody (Capture) (HA723901) and Recombinant Mouse IL-17F protein (HA211035) as the standard. The reference range value is 3.9-1,000 pg/mL. Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH17-16] to Mouse IL-17F antibody (Detector) (HA723903) and Recombinant Mouse IL-17F protein (HA211035) as the standard. The reference range value is 7.8-1,000 pg/mL. |
| Uniprot #: | SwissProt: Q7TNI7 Mouse |
| Alternative names: | CANDF6 Cytokine ML 1 Cytokine ML-1 IL 17F IL 24 IL-17F IL-24 Il17f Interleukin 17F Interleukin 24 Interleukin-17F Interleukin-24 ML 1 ML1 Mutant IL 17F OTTHUMP00000016602 |
Images
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Fig1:
Sandwich ELISA analysis of Mouse IL-17F matched pair antibodies Capture: HA723901, Mouse IL-17F Rabbit mAb [PSH17-14] Detector: HA723902, Mouse IL-17F Rabbit mAb [PSH17-15] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723901) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-17F protein (HA211035) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA723902, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Sandwich ELISA analysis of Mouse IL-17F matched pair antibodies Capture: HA723902, Mouse IL-17F Rabbit mAb [PSH17-15] Detector: HA723903, Mouse IL-17F Rabbit mAb [PSH17-16] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723902) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-17F protein (HA211035) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA723903, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig3:
Interpolated concentrations of spiked IL-17F in cell culture media samples. Capture: HA723901, Mouse IL-17F Rabbit mAb [PSH17-14] Detector: HA723902, Mouse IL-17F Rabbit mAb [PSH17-15] The concentrations of IL-17F were measured in duplicates, interpolated from the IL-17F standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
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Fig4:
Interpolated concentrations of spiked IL-17F in cell culture media samples. Capture: HA723902, Mouse IL-17F Rabbit mAb [PSH17-15] Detector: HA723903, Mouse IL-17F Rabbit mAb [PSH17-16] The concentrations of IL-17F were measured in duplicates, interpolated from the IL-17F standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.