Mouse EPO/Erythropoietin Recombinant Rabbit Monoclonal Antibody [PSH17-23]
cat.: HA723910
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH17-23 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse EPO aa 27-192 (HA211068). |
| Positive control: | Recombinant Mouse EPO/Erythropoietin protein (HA211068). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH17-22] to Mouse EPO/Erythropoietin antibody (Detector) (HA723909) and Recombinant Mouse EPO/Erythropoietin protein (HA211068) as the standard. The reference range value is 7.8-2,000 pg/mL. |
| Uniprot #: | SwissProt: P07321 Mouse |
| Alternative names: | EP EPO alpha EPO Epoetin Erythropoetin Erythropoietin precursor MVCD2 |
Images
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Fig1:
Sandwich ELISA analysis of Mouse EPO/Erythropoietin matched pair antibodies Capture: HA723910, Mouse EPO/Erythropoietin Rabbit mAb [PSH17-23] Detector: HA723909, Mouse EPO/Erythropoietin Rabbit mAb [PSH17-22] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723910) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse EPO/Erythropoietin protein (HA211068) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723909, Biotin, 0.1 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native Erythropoietin in mouse Balb/C serum samples. Capture: HA723910, Mouse EPO/Erythropoietin Rabbit mAb [PSH17-23] Detector: HA723909, Mouse EPO/Erythropoietin Rabbit mAb [PSH17-22] The concentrations of Erythropoietin were measured in duplicates, interpolated from the Erythropoietin standard curve and corrected for sample dilution. Undiluted samples are Balb/C serum 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Erythropoietin concentration was determined to be 132.7 pg/ml in Balb/C serum. |
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Fig3:
Interpolated concentrations of spiked Erythropoietin in mouse cell culture media samples. Capture: HA723910, Mouse EPO/Erythropoietin Rabbit mAb [PSH17-23] Detector: HA723909, Mouse EPO/Erythropoietin Rabbit mAb [PSH17-22] The concentrations of Erythropoietin were measured in duplicates, interpolated from the Erythropoietin standard curves and corrected for sample dilution. Diluted samples are as follows: 25% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.