ELKS Recombinant Rabbit Monoclonal Antibody [PSH17-33]
cat.: HA723916
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH17-33 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 128 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human ELKS aa 1-450. |
| Positive control: | HeLa cell lysate, Huh7 cell lysate, U-87 MG cell lysate, NIH/3T3 cell lysate, Neuro-2a cell lysate, PC-12 cell lysate, C6 cell lysate, Mouse brain tissue lysate, Mouse testis tissue lysate, Mouse kidney tissue lysate, Rat brain tissue lysate, Rat testis tissue lysate, Rat kidney tissue lysate. |
| Subcellular location: | Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Membrane, Golgi apparatus membrane, Presynaptic cell membrane, Cell projection, podosome. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: Q8IUD2 Human | Q99MI1 Mouse | Q811U3 Rat |
| Alternative names: | Cast 2 CAST2 CAZ associated structural protein 2 Elks ELKS/RAB6 interacting/CAST family member 1 ELKS/RAB6-interacting/CAST family member 1 ERC 1 ERC protein 1 ERC-1 Erc1 ERC1 protein KIAA1081 MGC12974 RAB6 interacting protein 2 RAB6-interacting protein 2 RAB6IP2 RB6I2_HUMAN |
Images
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Fig1:
Western blot analysis of ELKS on different lysates with Rabbit anti-ELKS antibody (HA723916) at 1/5,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: Huh7 cell lysate (20 µg/Lane) Lane 3: U-87 MG cell lysate (20 µg/Lane) Lane 4: NIH/3T3 cell lysate (20 µg/Lane) Lane 5: Neuro-2a cell lysate (20 µg/Lane) Lane 6: PC-12 cell lysate (20 µg/Lane) Lane 7: C6 cell lysate (20 µg/Lane) Lane 8: Mouse brain tissue lysate (40 µg/Lane) Lane 9: Mouse testis tissue lysate (40 µg/Lane) Lane 10: Mouse kidney tissue lysate (40 µg/Lane) Lane 11: Rat brain tissue lysate (40 µg/Lane) Lane 12: Rat testis tissue lysate (40 µg/Lane) Lane 13: Rat kidney tissue lysate (40 µg/Lane) Predicted band size: 128 kDa Observed band size: 120/130 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723916) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.