TMS1 / ASC Recombinant Rabbit Monoclonal Antibody [PSH17-34]
cat.: HA723917
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-Fr, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH17-34 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 21 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse TMS1 / ASC aa 1-193. |
| Positive control: | Mouse spleen tissue lysate, Mouse colon tissue lysate, HL-60 cell lysate, THP-1 cell lysate, U-937 cell lysate, human spleen tissue, mouse spleen tissue. |
| Subcellular location: | Cytoplasm, Inflammasome, Endoplasmic reticulum, Mitochondrion, Nucleus. |
| Recommended Dilutions:
WB IHC-Fr IHC-P |
1:5,000 1:500 1:200-1:1,000 |
| Uniprot #: | SwissProt: Q9ULZ3 Human | Q9EPB4 Mouse |
| Alternative names: | Apoptosis associated speck like protein containing a CARD Apoptosis-associated speck-like protein containing a CARD ASC CARD 5 CARD5 Caspase recruitment domain containing protein 5 Caspase recruitment domain protein 5 Caspase recruitment domain-containing protein 5 hASC MGC10332 PYCARD PYD and CARD domain containing PYD and CARD domain containing protein PYD and CARD domain-containing protein Target of methylation induced silencing 1 Target of methylation-induced silencing 1 TMS 1 TMS TMS1 |
Images
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Fig1:
Western blot analysis of TMS1 / ASC on different lysates with Rabbit anti-TMS1 / ASC antibody (HA723917) at 1/5,000 dilution. Lane 1: Mouse spleen tissue lysate Lane 2: Mouse colon tissue lysate Lane 3: Mouse skeletal muscle tissue lysate (low expression) Lysates/proteins at 30 µg/Lane. Predicted band size: 21 kDa Observed band size: 25 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723917) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of TMS1 / ASC on different lysates with Rabbit anti-TMS1 / ASC antibody (HA723917) at 1/5,000 dilution. Lane 1: HL-60 cell lysate Lane 2: THP-1 cell lysate Lane 3: U-937 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 21 kDa Observed band size: 25 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723917) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Application: IHC-Fr Species: Mouse Site: colon Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-TMS1 / ASC antibody (HA723917) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723917) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-TMS1 / ASC antibody (HA723917) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723917) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue (low expression) with Rabbit anti-TMS1 / ASC antibody (HA723917) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723917) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.