Biotin Conjugated Mouse Osteopontin Recombinant Rabbit Monoclonal Antibody [PSH17-50]
cat.: HA723925B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH17-50 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse Osteopontin aa 17-294 (HA210997). |
| Positive control: | Recombinant Mouse Osteopontin protein (HA210997). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH17-49] to Mouse Osteopontin antibody (Capture) (HA723924) and Recombinant Mouse Osteopontin protein (HA210997) as the standard. The reference range value is 9.8-2,500 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P10923 Mouse |
| Alternative names: | BNSP Bone sialoprotein 1 Bone sialoprotein I BSP I BSPI Early T lymphocyte activation 1 ETA 1 ETA1 MGC110940 Nephropontin OPN Osteopontin osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein PSEC0156 secreted phosphoprotein 1 (osteopontin bone sialoprotein I early T lymphocyte activation 1) secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1) Secreted phosphoprotein 1 SPP 1 SPP-1 SPP1 SPP1/CALPHA1 fusion Urinary stone protein Uropontin |
Images
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Fig1:
Sandwich ELISA analysis of Mouse Osteopontin matched pair antibodies Capture: HA723924, Mouse Osteopontin Rabbit mAb [PSH17-49] Detector: HA723925, Mouse Osteopontin Rabbit mAb [PSH17-50] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723924) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Osteopontin protein (HA210997) protein starting from 2,500 pg/ml to 0 pg/ml and detect antibody (HA723925, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native spp1 in RAW 264.7 cell extract samples based on a 1000 µg/ml extract load. Capture: HA723924, Mouse Osteopontin Rabbit mAb [PSH17-49] Detector: HA723925, Mouse Osteopontin Rabbit mAb [PSH17-50] Interpolated concentration of native spp1 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean spp1 concentration was determined to be 79,438,854 pg/ml in RAW 264.7 treated cell extract and 6,041,158 pg/ml in RAW 264.7 untreated cell extract. |
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Fig3:
Interpolated concentrations of native spp1 in Mouse serum. Capture: HA723924, Mouse Osteopontin Rabbit mAb [PSH17-49] Detector: HA723925, Mouse Osteopontin Rabbit mAb [PSH17-50] Interpolated concentration of native spp1 was measured in duplicate at different sample concentrations and interpolated from the spp1 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean spp1 concentration was determined to be 77,206 pg/ml in Mouse serum. |
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Fig4:
Interpolated concentrations of native spp1 in J774A.1 cell culture supernatant. Capture: HA723924, Mouse Osteopontin Rabbit mAb [PSH17-49] Detector: HA723925, Mouse Osteopontin Rabbit mAb [PSH17-50] Interpolated concentration of native spp1 was measured in duplicate at different sample concentrations and interpolated from the spp1 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean spp1 concentration was determined to be 92,201 pg/ml in J774A.1 cell culture supernatant. |
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Fig5:
Interpolated concentrations of spiked spp1 in cell culture media samples. Capture: HA723924, Mouse Osteopontin Rabbit mAb [PSH17-49] Detector: HA723925, Mouse Osteopontin Rabbit mAb [PSH17-50] The concentrations of spp1 were measured in duplicates, interpolated from the spp1 standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.