HRP Conjugated Mouse Osteopontin Recombinant Rabbit Monoclonal Antibody [PSH17-50]
cat.: HA723925H
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH17-50 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse Osteopontin aa 17-294 (HA210997). |
| Positive control: | Recombinant Mouse Osteopontin protein (HA210997). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH17-49] to Mouse Osteopontin antibody (Capture) (HA723924) and Recombinant Mouse Osteopontin protein (HA210997) as the standard. The reference range value is 3,390-12,500 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P10923 Mouse |
| Alternative names: | BNSP Bone sialoprotein 1 Bone sialoprotein I BSP I BSPI Early T lymphocyte activation 1 ETA 1 ETA1 MGC110940 Nephropontin OPN Osteopontin osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein PSEC0156 secreted phosphoprotein 1 (osteopontin bone sialoprotein I early T lymphocyte activation 1) secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1) Secreted phosphoprotein 1 SPP 1 SPP-1 SPP1 SPP1/CALPHA1 fusion Urinary stone protein Uropontin |
Images
|
Fig1:
Sandwich ELISA analysis of Mouse Osteopontin matched pair antibodies Capture: HA723924, Mouse Osteopontin Rabbit mAb [PSH17-49] Detector: HA723925, Mouse Osteopontin Rabbit mAb [PSH17-50] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723924) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Osteopontin protein (HA210997) protein starting from 12,500 pg/ml to 0 pg/ml and detect antibody (HA723925, HRP, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.