Rat ICAM1 Recombinant Rabbit Monoclonal Antibody [PSH17-52]
cat.: HA723927
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Rat
Applications: ELISA(Det)
Clonality: Monoclonal
Clone number: PSH17-52
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4).
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Isotype: IgG
Immunogen: Recombinant protein within Rat ICAM1 aa 28-493 (HA211063).
Positive control: Recombinant Rat ICAM1 protein (HA211063).
Subcellular location: Membrane.
Recommended Dilutions:
  ELISA(Det)
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH17-51] to Rat ICAM1 antibody (Capture) (HA723926) or Rabbit monoclonal [PSH17-53] to Rat ICAM1 antibody (Capture) (HA723928) and Recombinant Rat ICAM1 protein (HA211063) as the standard. The reference range value is 31.3-4,000 pg/mL.
Uniprot #: SwissProt: Q00238 Rat
Alternative names: Antigen identified by monoclonal BB2 BB 2 BB2 CD 54 CD_antigen=CD54 CD54 Cell surface glycoprotein P3.58 Human rhinovirus receptor ICAM 1 ICAM-1 ICAM1 ICAM1_HUMAN intercellular adhesion molecule 1 (CD54) intercellular adhesion molecule 1 (CD54), human rhinovirus receptor Intercellular adhesion molecule 1 Major group rhinovirus receptor MALA 2 MALA2 MyD 10 MyD10 P3.58 Surface antigen of activated B cells Surface antigen of activated B cells, BB2
Images
HA723927_1.png Fig1: Sandwich ELISA analysis of Rat ICAM1 matched pair antibodies

Capture: HA723926, Rat ICAM1 Rabbit mAb [PSH17-51]
Detector: HA723927, Rat ICAM1 Rabbit mAb [PSH17-52]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723926) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Rat ICAM1 protein (HA211063) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723927, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
HA723927_2.png Fig2: Sandwich ELISA analysis of Rat ICAM1 matched pair antibodies

Capture: HA723928, Rat ICAM1 Rabbit mAb [PSH17-53]
Detector: HA723927, Rat ICAM1 Rabbit mAb [PSH17-52]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723928) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Rat ICAM1 protein (HA211063) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723927, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
HA723927_3.png Fig3: Interpolated concentrations of native ICAM1 in rat serum samples.

Capture: HA723926, Rat ICAM1 Rabbit mAb [PSH17-51]
Detector: HA723927, Rat ICAM1 Rabbit mAb [PSH17-52]

The concentrations of ICAM1 were measured in duplicates, interpolated from the ICAM1 standard curve and corrected for sample dilution. Undiluted samples are rat serum 2.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ICAM1 concentration was determined to be 62,341 pg/ml in rat serum.
HA723927_4.png Fig4: Interpolated concentrations of native ICAM1 in rat serum samples.

Capture: HA723928, Rat ICAM1 Rabbit mAb [PSH17-53]
Detector: HA723927, Rat ICAM1 Rabbit mAb [PSH17-52]

The concentrations of ICAM1 were measured in duplicates, interpolated from the ICAM1 standard curve and corrected for sample dilution. Undiluted samples are rat serum 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ICAM1 concentration was determined to be 21,617 pg/ml in rat serum.
HA723927_5.png Fig5: Interpolated concentrations of spiked ICAM1 in rat cell culture media samples.

Capture: HA723926, Rat ICAM1 Rabbit mAb [PSH17-51]
Detector: HA723927, Rat ICAM1 Rabbit mAb [PSH17-52]

The concentrations of ICAM1 were measured in duplicates, interpolated from the ICAM1 standard curves and corrected for sample dilution. Diluted samples are as follows: 25% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
HA723927_6.png Fig6: Interpolated concentrations of spiked ICAM1 in rat cell culture media samples.

Capture: HA723928, Rat ICAM1 Rabbit mAb [PSH17-53]
Detector: HA723927, Rat ICAM1 Rabbit mAb [PSH17-52]

The concentrations of ICAM1 were measured in duplicates, interpolated from the ICAM1 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.