HRP Conjugated Rat ICAM1 Recombinant Rabbit Monoclonal Antibody [PSH17-52]
cat.: HA723927H
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Rat |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH17-52 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Rat ICAM1 aa 28-493 (HA211063). |
| Positive control: | Recombinant Rat ICAM1 protein (HA211063). |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH17-51] to Rat ICAM1 antibody (Capture) (HA723926) or Rabbit monoclonal [PSH17-53] to Rat ICAM1 antibody (Capture) (HA723928) and Recombinant Rat ICAM1 protein (HA211063) as the standard. The reference range value is 625-80,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q00238 Rat |
| Alternative names: | Antigen identified by monoclonal BB2 BB 2 BB2 CD 54 CD_antigen=CD54 CD54 Cell surface glycoprotein P3.58 Human rhinovirus receptor ICAM 1 ICAM-1 ICAM1 ICAM1_HUMAN intercellular adhesion molecule 1 (CD54) intercellular adhesion molecule 1 (CD54), human rhinovirus receptor Intercellular adhesion molecule 1 Major group rhinovirus receptor MALA 2 MALA2 MyD 10 MyD10 P3.58 Surface antigen of activated B cells Surface antigen of activated B cells, BB2 |
Images
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Fig1:
Sandwich ELISA analysis of Rat ICAM1 matched pair antibodies Capture: HA723928, Rat ICAM1 Rabbit mAb [PSH17-53] Detector: HA723927, Rat ICAM1 Rabbit mAb [PSH17-52] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723928) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Rat ICAM1 protein (HA211063) starting from 80,000 pg/ml to 0 pg/ml and detect antibody (HA723927, HRP, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.