PDE1B Recombinant Rabbit Monoclonal Antibody [PSH17-59]
cat.: HA723934
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB, IHC-P, IHC-Fr |
| Clonality: | Monoclonal |
| Clone number: | PSH17-59 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 61 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PDE1B aa 401-536. |
| Positive control: | Mouse brain tissue lysate, Rat brain tissue lysate, Rat cerebellum tissue lysate, Rat hippocampus tissue lysate, mouse cerebrum tissue, mouse corpus striatum tissue, rat cerebellum tissue. |
| Subcellular location: | Cytoplasm, cytosol. |
| Recommended Dilutions:
WB IHC-P IHC-Fr |
1:5,000 1:1,000 1:500 |
| Uniprot #: | SwissProt: Q01065 Mouse | Q01066 Rat |
| Alternative names: | 5''-cyclic nucleotide phosphodiesterase 1B 63 kDa Cam PDE 63 kDa Cam-PDE Calcium/calmodulin dependent 3' 5' cyclic nucleotide Phosphodiesterase 1B Calcium/calmodulin stimulated cyclic nucleotide phosphodiesterase Calcium/calmodulin-dependent 3'' Calmodulin stimulated phosphodiesterase PDE1B1 Cam PDE 1B Cam PDE1B Cam-PDE 1B PDE 1B PDE1B PDE1B_HUMAN PDE1B1 PDES 1B PDES1B Phosphodiesterase 1B Phosphodiesterase 1B calmodulin dependent Presumed 63kDa form of the type 1 cyclic nucleotide phosphodiesterase family known as PDE1B |
Images
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Fig1:
Western blot analysis of PDE1B on different lysates with Rabbit anti-PDE1B antibody (HA723934) at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate (10 µg/Lane) Lane 2: Rat brain tissue lysate (10 µg/Lane) Lane 3: Rat cerebellum tissue lysate (30 µg/Lane) Lane 4: Rat hippocampus tissue lysate (20 µg/Lane) Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723934) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue with Rabbit anti-PDE1B antibody (HA723934) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723934) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse corpus striatum tissue with Rabbit anti-PDE1B antibody (HA723934) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723934) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-PDE1B antibody (HA723934) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723934) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Application: IHC-Fr Species: Mouse Site: brain (striatum) Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.