OSMR Recombinant Rabbit Monoclonal Antibody [PSH17-60]
cat.: HA723935
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Monoclonal
Clone number: PSH17-60
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 111 kDa
Isotype: IgG
Immunogen: Recombinant protein within human OSMR aa 1-740.
Positive control: HeLa cell lysate, HCT 116 cell lysate.
Subcellular location: Membrane.
Recommended Dilutions:
  WB

1:5,000
Uniprot #: SwissProt: Q99650 Human
Alternative names: IL-31 receptor subunit beta IL-31R subunit beta IL-31R-beta IL-31RB Interleukin-31 receptor subunit beta MGC140467 MGC150626 MGC150627 MGC75127 Oncostatin M receptor Oncostatin M specific receptor subunit beta Oncostatin M-specific receptor, beta Oncostatin-M-specific receptor subunit beta Osmr OSMR_HUMAN OSMRB
Images
HA723935_1.jpg Fig1: Western blot analysis of OSMR on different lysates with Rabbit anti-OSMR antibody (HA723935) at 1/5,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: Jurkat cell lysate (negative)
Lane 3: HCT 116 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 111 kDa
Observed band size: 150-180 kDa

Exposure time: 3 minutes; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723935) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.