| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH17-60 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 111 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human OSMR aa 1-740. |
| Positive control: | HeLa cell lysate, HCT 116 cell lysate. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: Q99650 Human |
| Alternative names: | IL-31 receptor subunit beta IL-31R subunit beta IL-31R-beta IL-31RB Interleukin-31 receptor subunit beta MGC140467 MGC150626 MGC150627 MGC75127 Oncostatin M receptor Oncostatin M specific receptor subunit beta Oncostatin M-specific receptor, beta Oncostatin-M-specific receptor subunit beta Osmr OSMR_HUMAN OSMRB |
|
Fig1:
Western blot analysis of OSMR on different lysates with Rabbit anti-OSMR antibody (HA723935) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate (negative) Lane 3: HCT 116 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 111 kDa Observed band size: 150-180 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723935) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |