CD200 / OX2 Recombinant Rabbit Monoclonal Antibody [PSH17-62]
cat.: HA723937
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | IHC-Fr, IHC-P, WB |
| Clonality: | Monoclonal |
| Clone number: | PSH17-62 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 31 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse CD200 aa 1-232. |
| Positive control: | Mouse brain tissue, mouse spleen tissue, Mouse brain tissue lysate, Mouse lung tissue lysate. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
IHC-Fr IHC-P WB |
1:500 1:1,000 1:5,000 |
| Uniprot #: | SwissProt: O54901 Mouse |
| Alternative names: | Antigen identified by monoclonal MRC OX 2 CD200 CD200 antigen CD200 molecule MOX1 MOX2 MRC MRC OX 2 antigen My033 OX 2 OX 2 membrane glycoprotein precursor OX-2 membrane glycoprotein OX2G |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: brain Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-CD200 / OX2 antibody (HA723937) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723937) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-CD200 / OX2 antibody (HA723937) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723937) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Western blot analysis of CD200 / OX2 on different lysates with Rabbit anti-CD200 / OX2 antibody (HA723937) at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse lung tissue lysate Lane 3: Mouse liver tissue lysate (low expression) Lysates/proteins at 30 µg/Lane. Predicted band size: 31 kDa Observed band size: 40-50 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723937) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.