Mouse DKK1 Recombinant Rabbit Monoclonal Antibody [PSH17-70]
cat.: HA723939
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH17-70 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse DKK1 aa 32-272 (HA211082). |
| Positive control: | Recombinant Mouse DKK1 protein (HA211082). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH17-69] to Mouse DKK1 antibody (Capture) (HA723938) and Recombinant Mouse DKK1 protein (HA211082) as the standard. The reference range value is 78.1-5,000 pg/mL. |
| Uniprot #: | SwissProt: O54908 Mouse |
| Alternative names: | Dickkopf 1 Dickkopf 1 homolog Dickkopf 1 like Dickkopf homolog 1 Dickkopf like protein 1 Dickkopf related protein 1 Dickkopf WNT signaling pathway inhibitor 1 Dickkopf-1 Dickkopf-related protein 1 DKK 1 Dkk-1 Dkk1 hDkk 1 hDkk-1 SK |
Images
|
Fig1:
Sandwich ELISA analysis of Mouse DKK1 matched pair antibodies Capture: HA723938, Mouse DKK1 Rabbit mAb [PSH17-69] Detector: HA723939, Mouse DKK1 Rabbit mAb [PSH17-70] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723938) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse DKK1 protein (HA211082) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA723939, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native DKK1 in Mouse serum. Capture: HA723938, Mouse DKK1 Rabbit mAb [PSH17-69] Detector: HA723939, Mouse DKK1 Rabbit mAb [PSH17-70] Interpolated concentration of native DKK1 was measured in duplicate at different sample concentrations and interpolated from the DKK1 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean DKK1 concentration was determined to be 11,339 pg/ml in Mouse serum. |
|
Fig3:
Interpolated concentrations of native DKK1 in Neuro-2a and C2C12 cell culture supernatant. Capture: HA723938, Mouse DKK1 Rabbit mAb [PSH17-69] Detector: HA723939, Mouse DKK1 Rabbit mAb [PSH17-70] Interpolated concentration of native DKK1 was measured in duplicate at different sample concentrations and interpolated from the DKK1 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean DKK1 concentration was determined to be 20,871 pg/ml in Neuro-2a cell culture supernatant and undetectable in C2C12 cell culture supernatant. |
|
Fig4:
Interpolated concentrations of spiked DKK1 in cell culture media samples. Capture: HA723938, Mouse DKK1 Rabbit mAb [PSH17-69] Detector: HA723939, Mouse DKK1 Rabbit mAb [PSH17-70] The concentrations of DKK1 were measured in duplicates, interpolated from the DKK1 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.