Mouse Apolipoprotein B/Apo B Recombinant Rabbit Monoclonal Antibody [PSH17-75]
cat.: HA723944
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH17-75 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse Apolipoprotein B aa 401-810 (HA211327). |
| Positive control: | Recombinant Mouse Apolipoprotein B/Apo B protein (HA211327). |
| Subcellular location: | Cytoplasm, Secreted, Lipid droplet. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH17-76] to Mouse Apolipoprotein B/Apo B antibody (Detector) (HA723945) and Recombinant Mouse Apolipoprotein B/Apo B protein (HA211327) as the standard. The reference range value is 15.6-4,000 pg/mL. |
| Uniprot #: | SwissProt: E9Q414 Mouse |
| Alternative names: | Apo B 100 Apo B Apo B-100 Apo B-48 Apo B100 Apo B48 ApoB 100 ApoB 48 APOB Apolipoprotein B (including Ag(x) antigen) Apolipoprotein B 100 Apolipoprotein B 48 Apolipoprotein B Apolipoprotein B-48 Apolipoprotein B100 Apolipoprotein B48 FLDB LDLCQ4 |
Images
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Fig1:
Sandwich ELISA analysis of Mouse Apolipoprotein B/Apo B matched pair antibodies Capture: HA723944, Mouse Apolipoprotein B/Apo B Rabbit mAb [PSH17-75] Detector: HA723945, Mouse Apolipoprotein B/Apo B Rabbit mAb [PSH17-76] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723944) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Apolipoprotein B/Apo B protein (HA211327) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723945, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native Apolipoprotein B in mouse samples. Capture: HA723944, Mouse Apolipoprotein B/Apo B Rabbit mAb [PSH17-75] Detector: HA723945, Mouse Apolipoprotein B/Apo B Rabbit mAb [PSH17-76] Interpolated concentration of native Apolipoprotein B was measured in duplicate at different sample concentrations and interpolated from the Apolipoprotein B standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Apolipoprotein B concentration was determined to be 84.467 ug/mL in Mouse serum. There was no detectable signal in NIH/3T3 cell supernatant. This immunoassay is calibrated against highly purified ApoB from human plasma. |
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Fig3:
Interpolated concentrations of spiked Apolipoprotein B in cell culture media samples. Capture: HA723944, Mouse Apolipoprotein B/Apo B Rabbit mAb [PSH17-75] Detector: HA723945, Mouse Apolipoprotein B/Apo B Rabbit mAb [PSH17-76] The concentrations of Apolipoprotein B were measured in duplicates, interpolated from the Apolipoprotein B standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.