c-Kit Recombinant Rabbit Monoclonal Antibody [PSH17-79]
cat.: HA723948
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | IHC-Fr, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH17-79 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 109 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse c-Kit aa 1-527. |
| Positive control: | Mouse testis tissue, ES-D3. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
IHC-Fr IF-Cell FC |
1:500 1:50 1:1,000 |
| Uniprot #: | SwissProt: P05532 Mouse |
| Alternative names: | C Kit c-Kit c-Kit Ligand CD117 Kit Kit Ligand KIT oncogene KIT proto oncogene receptor tyrosine kinase Mast cell growth factor receptor Mast/stem cell growth factor receptor Kit MGF p145 c-kit PBT Piebald trait protein Proto oncogene c Kit Proto oncogene tyrosine protein kinase Kit Proto-oncogene c-Kit SCF Receptor SCFR soluble KIT variant 1 Steel Factor Receptor Stem cell factor receptor tyrosine protein kinase Kit Tyrosine-protein kinase Kit v kit Hardy Zuckerman 4 feline sarcoma viral oncogene homolog v kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: testis Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
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Fig2:
Immunocytochemistry analysis of ES-D3 cells labeling c-Kit with Rabbit anti-c-Kit antibody (HA723948) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-c-Kit antibody (HA723948) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
|
Fig3:
Flow cytometric analysis of NIH/3T3 (left, negative) and ES-D3 (right, positive) cells labeling c-Kit. Cells were fixed and permeabilized. Then stained with the primary antibody (HA723948, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.