Mouse Prolactin Recombinant Rabbit Monoclonal Antibody [PSH17-90]
cat.: HA723955
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH17-90 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse Prolactin aa 30-226 (HA211036). |
| Positive control: | Recombinant Mouse Prolactin protein (HA211036). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH17-91] to Mouse Prolactin antibody (Detector) (HA723956) and Recombinant Mouse Prolactin protein (HA211036) as the standard. The reference range value is 46.9-6,000 pg/mL. |
| Uniprot #: | SwissProt: P06879 Mouse |
| Alternative names: | Decidual prolactin GHA1 Growth hormone A1 Lactogenic hormone Luteotropic hormone Mammotropin PRL Prolactin Prolactin precursor |
Images
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Fig1:
Sandwich ELISA analysis of Mouse Prolactin matched pair antibodies Capture: HA723955, Mouse Prolactin Rabbit mAb [PSH17-90] Detector: HA723956, Mouse Prolactin Rabbit mAb [PSH17-91] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723955) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Prolactin protein (HA211036) starting from 6,000 pg/ml to 0 pg/ml and detect antibody (HA723956, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native Prolactin in Mouse serum. Capture: HA723955, Mouse Prolactin Rabbit mAb [PSH17-90] Detector: HA723956, Mouse Prolactin Rabbit mAb [PSH17-91] Interpolated concentration of native Prolactin was measured in duplicate at different sample concentrations and interpolated from the Prolactin standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Prolactin concentration was determined to be 4,029 pg/ml in normal Mouse serum and 24,039 pg/ml in pregnant Mouse serum. |
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Fig3:
Interpolated concentrations of spiked Prolactin in cell culture media samples. Capture: HA723955, Mouse Prolactin Rabbit mAb [PSH17-90] Detector: HA723956, Mouse Prolactin Rabbit mAb [PSH17-91] The concentrations of Prolactin were measured in duplicates, interpolated from the Prolactin standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.