c-Kit Recombinant Rabbit Monoclonal Antibody [PSH17-96]
cat.: HA723961
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB, IHC-Fr, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH17-96 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 109 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse c-Kit aa 1-527. |
| Positive control: | ES-D3 cell lysate, Mouse brain tissue lysate, Mouse testis tissue lysate, Rat brain tissue lysate, Rat cerebellum tissue lysate, rat cerebellum tissue, rat colon tissue, rat testis tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-Fr IHC-P |
1:5,000 1:500 1:200-1:5,000 |
| Uniprot #: | SwissProt: P05532 Mouse Entrez Gene: 64030 Rat |
| Alternative names: | C Kit c-Kit c-Kit Ligand CD117 Kit Kit Ligand KIT oncogene KIT proto oncogene receptor tyrosine kinase Mast cell growth factor receptor Mast/stem cell growth factor receptor Kit MGF p145 c-kit PBT Piebald trait protein Proto oncogene c Kit Proto oncogene tyrosine protein kinase Kit Proto-oncogene c-Kit SCF Receptor SCFR soluble KIT variant 1 Steel Factor Receptor Stem cell factor receptor tyrosine protein kinase Kit Tyrosine-protein kinase Kit v kit Hardy Zuckerman 4 feline sarcoma viral oncogene homolog v kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog |
Images
|
Fig1:
Western blot analysis of c-Kit on different lysates with Rabbit anti-c-Kit antibody (HA723961) at 1/5,000 dilution. Lane 1: ES-D3 cell lysate (20 µg/Lane) Lane 2: NIH/3T3 cell lysate (negative) (20 µg/Lane) Lane 3: Mouse brain tissue lysate (20 µg/Lane) Lane 4: Mouse testis tissue lysate (20 µg/Lane) Lane 5: Rat brain tissue lysate (20 µg/Lane) Lane 6: Rat cerebellum tissue lysate (20 µg/Lane) Predicted band size: 109 kDa Observed band size: 140 kDa Exposure time: 42 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723961) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Application: IHC-Fr Species: Rat Site: cerebellum Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-c-Kit antibody (HA723961) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723961) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-c-Kit antibody (HA723961) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723961) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-c-Kit antibody (HA723961) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723961) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.