FGL1 Recombinant Rabbit Monoclonal Antibody [PSH17-97]
cat.: HA723962
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH17-97 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 36 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human FGL1 aa 1-312. |
| Positive control: | HepG2 cell lysate. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IP |
1:5,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q08830 Human |
| Alternative names: | FGL 1 Fgl1 FGL1_HUMAN Fibrinogen like 1 Fibrinogen like protein 1 Fibrinogen related protein 1 Fibrinogen-like protein 1 Hepassocin Hepatocellular carcinoma related sequence Hepatocyte derived fibrinogen related protein 1 Hepatocyte-derived fibrinogen-related protein 1 HFREP 1 HFREP-1 HFREP1 HP 041 HP-041 HP041 LFIRE 1 LFIRE-1 LFIRE1 Liver fibrinogen related protein 1 Liver fibrinogen-related protein 1 MFIRE 1 MGC108569 MGC12455 MGC37822 OTTHUMP00000122468 |
Images
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Fig1:
Western blot analysis of FGL1 on different lysates with Rabbit anti-FGL1 antibody (HA723962) at 1/5,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HCT 116 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 36 kDa Observed band size: 33 kDa Exposure time: 1 minute; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723962) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
FGL1 was immunoprecipitated from 0.2 mg HepG2 cell lysate with HA723962 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723962 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HepG2 cell lysate (input) Lane 2: HA723962 IP in HepG2 cell lysate Lane 3: Rabbit IgG instead of HA723962 in HepG2 cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 10 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.